Ambrosino D M, Delaney N R, Shamberger R C
Laboratory of Infectious Diseases, Dana-Farber Cancer Institute, Boston, MA 02115.
J Immunol. 1990 Feb 15;144(4):1221-6.
The responsiveness of polysaccharide-specific B cells to PWM was examined in vitro. Spleen cells from six patients immunized with Haemophilus influenzae type b-diphtheria toxoid, pneumococcal and meningococcal vaccines were T cell-depleted and separated by Percoll density gradient centrifugation. In each B cell fraction, spontaneous antibody production was demonstrated to capsular polysaccharides as well as diphtheria toxoid. The peak of spontaneous antibody production was demonstrated to be five to seven days after immunization. When T cells and PWM were added, the total Ig secretion increased in all B cell fractions. PWM also enhanced IgG antibody directed to each of three polysaccharide Ag measured. This enhancement was most noticeable for nonresting B cells. The PWM effect was not confined to IgG, as IgM and IgA to Neisseria meningitidis type C were measured and also enhanced. The kinetics of the PWM response demonstrated the most IgG antibody to polysaccharide Ag from spleens immunized five to seven days before splenectomy. When the patients were immunized either 2 days or 4 mo before splenectomy, no spontaneous IgG antibody to polysaccharides was detected although PWM induced small amounts of antibody. Finally, anti-IL-6 antibody blocked PWM-induced total and polysaccharide-specific antibody production. We conclude that human polysaccharide-specific B cells are responsive to PWM and IL-6. We suggest that polysaccharide B cells are not truly "T cell-independent" and may respond to T cell lymphokines and thus are similar to protein-specific B cells.
在体外检测了多糖特异性B细胞对PWM的反应性。对6例接种b型流感嗜血杆菌-白喉类毒素、肺炎球菌和脑膜炎球菌疫苗的患者的脾细胞进行T细胞去除，并通过Percoll密度梯度离心进行分离。在每个B细胞组分中，均证实对荚膜多糖以及白喉类毒素有自发抗体产生。自发抗体产生的峰值出现在免疫后5至7天。当加入T细胞和PWM时，所有B细胞组分中的总Ig分泌均增加。PWM还增强了针对所检测的三种多糖抗原中每一种的IgG抗体。这种增强在非静止B细胞中最为明显。PWM的作用并不局限于IgG，因为对C群脑膜炎奈瑟菌的IgM和IgA也进行了检测并且也有所增强。PWM反应的动力学表明，脾切除术前5至7天免疫的脾脏中，针对多糖抗原的IgG抗体最多。当患者在脾切除术前2天或4个月进行免疫时，尽管PWM诱导产生少量抗体，但未检测到针对多糖的自发IgG抗体。最后，抗IL-6抗体阻断了PWM诱导的总抗体和多糖特异性抗体的产生。我们得出结论，人多糖特异性B细胞对PWM和IL-6有反应。我们认为多糖B细胞并非真正的“非T细胞依赖性”，可能对T细胞淋巴因子有反应，因此与蛋白质特异性B细胞相似。
