Lesse A J, Campagnari A A, Bittner W E, Apicella M A
Department of Medicine, State University of New York, Buffalo.
J Immunol Methods. 1990 Jan 24;126(1):109-17. doi: 10.1016/0022-1759(90)90018-q.
We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.
我们利用最近描述的三羟甲基氨基甲烷-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（TSDS-PAGE）系统来研究革兰氏阴性菌的脂寡糖（LOS）和脂多糖（LPS）。TSDS-PAGE在“微型凝胶”形式下实现了LOS和LPS的高度分辨率。TSDS-PAGE使LOS和LPS在电泳过程中根据其相对分子质量（Mr）迁移，并允许根据蛋白质标准品估计Mr。分析了几种LOS。新描述的方法能够对LOS和LPS的核心区域进行更快速、准确的分析。
