Griffiss J M, O'Brien J P, Yamasaki R, Williams G D, Rice P A, Schneider H
Infect Immun. 1987 Aug;55(8):1792-800. doi: 10.1128/iai.55.8.1792-1800.1987.
We studied the oligosaccharides (OS) of outer membrane lipooligosaccharides (LOS) of Neisseria gonorrhoeae and Neisseria meningitidis. OS from the LOS of an individual neisserial strain always eluted from Sephadex G-50S as multiple peaks; the polyacrylamide gel elution profiles were nearly identical to the polyacrylamide gel electrophoresis profiles of the sodium dodecyl sulfate-disaggregated native LOS from which the OS were derived. Neisserial OS coeluted with Dex14 to Dex27 dextran oligomers (Mr, 2,210 to 4,320). Monosaccharide composition varied among the several OS released from the LOS of a single strain. The two OS of a gonococcal strain sensitive to normal human serum (NHS) bacteriolysis (sers) varied in their ability to inhibit the binding of NHS immunoglobulin M to their parental LOS. The OS that was rich in hexosamines inhibited NHS immune lysis of its parent strain; the OS that was poor in hexosamines did not. We conclude that structural differences in their OS account for the Mr heterogeneity of the LOS of a strain.
我们研究了淋病奈瑟菌和脑膜炎奈瑟菌外膜脂寡糖（LOS）中的寡糖（OS）。单个奈瑟菌菌株LOS中的OS从Sephadex G - 50S上洗脱时总是呈现多个峰；聚丙烯酰胺凝胶洗脱图谱与从中衍生出OS的十二烷基硫酸钠解离的天然LOS的聚丙烯酰胺凝胶电泳图谱几乎相同。奈瑟菌OS与Dex14至Dex27葡聚糖寡聚物（Mr，2210至4320）共洗脱。从单个菌株的LOS释放的几种OS的单糖组成各不相同。对正常人血清（NHS）细菌溶解敏感的淋病奈瑟菌菌株（sers）的两种OS在抑制NHS免疫球蛋白M与其亲本LOS结合的能力上有所不同。富含己糖胺的OS抑制其亲本菌株的NHS免疫溶解；己糖胺含量低的OS则没有。我们得出结论，其OS的结构差异导致了菌株LOS的Mr异质性。
