Williams T P, Webbers J P
Appl Opt. 1995 Sep 1;34(25):5720-4. doi: 10.1364/AO.34.005720.
We describe a photometer that measures light transmitted through excised eyes. The instrument, an ocular transmission photometer, employs sensitive single photon-counting techniques, and its usefulness has been tested by the study of the absorbance of rhodopsin in retinal rod cells in situ. We find that absorbances of rat rods agree well with those predicted by microspectrophotometry without making corrections for cellular mosaics. Additional tests of the ocular transmission photometer show that (a) the instrument is sensitive to subtle differences in rhodopsin absorbance, known to exist in specific locations in the rat retina, and (b) using the rate of rhodopsin bleaching as the measure of intensity, we can determine the intensity distribution at several locations across the rat retina.
我们描述了一种用于测量透过离体眼睛的光的光度计。该仪器为眼透射光度计，采用了灵敏的单光子计数技术，并且通过对原位视网膜视杆细胞中视紫红质吸光度的研究测试了其效用。我们发现，大鼠视杆细胞的吸光度与微观分光光度法预测的结果非常吻合，无需对细胞镶嵌进行校正。眼透射光度计的其他测试表明：（a）该仪器对视紫红质吸光度的细微差异敏感，已知这种差异存在于大鼠视网膜的特定位置；（b）以视紫红质漂白速率作为强度度量，我们可以确定大鼠视网膜多个位置的强度分布。
