金黄色葡萄球菌的二进制分型。

Binary IS typing for Staphylococcus aureus.

机构信息

Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.

出版信息

PLoS One. 2010 Oct 27;5(10):e13671. doi: 10.1371/journal.pone.0013671.

BACKGROUND

We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus.

METHODOLOGY/PRINCIPAL FINDINGS: A novel approach of IS-typing was performed in which binary profiles are created. 424 human and animal derived MRSA and MSSA isolates were tested and a subset of these isolates was compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP). Binary IS typing had a high discriminatory potential and a good correlation with MLST and AFLP.

CONCLUSIONS/SIGNIFICANCE: Binary IS typing is easy to perform and binary profiles can be generated in a standardized fashion. These two features, combined with the high correlation with MLST clonal complexes, make the technique applicable for large-scale inter-laboratory molecular epidemiological comparisons.

背景

我们提出了一种简单易行的用于快速对金黄色葡萄球菌进行二元分型的检测方法：二元间隔（IS）分型。该检测方法是对先前描述的分子分型技术的进一步发展，该技术基于金黄色葡萄球菌 16S-23S rDNA 间隔区的长度多态性。

方法/主要发现：采用了一种新的 IS 分型方法，创建了二进制图谱。对 424 株来自人和动物的耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）分离株进行了检测，并将其中一部分分离株与多位点序列分型（MLST）和扩增片段长度多态性（AFLP）进行了比较。二元 IS 分型具有较高的分辨力，与 MLST 和 AFLP 相关性良好。

结论/意义：二元 IS 分型易于操作，并且可以标准化地生成二进制图谱。这两个特点，再加上与 MLST 克隆复合体的高度相关性，使得该技术适用于大规模的实验室间分子流行病学比较。