Herr Natalie R, Daniel Kevin B, Belle Anna M, Carelli Regina M, Wightman R Mark
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3290.
ACS Chem Neurosci. 2010 Jul 1;1(9):627-638. doi: 10.1021/cn100056r.
Iontophoresis allows for localized drug ejections directly into brain regions of interest driven by the application of current. Our lab has previously adapted a method to quantitatively monitor iontophoretic ejections. Here those principles have been applied in vivo to modulate electrically evoked release of dopamine in anesthetized rats. A neutral, electroactive marker molecule that is ejected purely by electroosmotic flow (EOF) was used to monitor indirectly the ejection of electroinactive dopaminergic drugs (raclopride, quinpirole, and nomifensine). Electrode placements were marked with an iontophoretically ejected dye, pontamine sky blue. We show that EOF marker molecules, acetaminophen (AP) and 2-(4-nitrophenoxy) ethanol, have no effect on electrically evoked dopamine release in the striatum or the sensitivity of electrode. Additionally, we establish that a short, 30 second ejection of raclopride, quinpirole, or nomifensine with iontophoresis is sufficient to affect autoreceptor regulation and the re-uptake of dopamine. These effects vary in lifetime, indicating that this technique can be used to study receptor kinetics.
离子电渗疗法可在电流作用下将药物直接局部注入感兴趣的脑区。我们实验室之前改进了一种方法来定量监测离子电渗注入。在此，这些原理已应用于活体实验，以调节麻醉大鼠中电诱发的多巴胺释放。一种仅通过电渗流（EOF）排出的中性电活性标记分子被用于间接监测电惰性多巴胺能药物（雷氯必利、喹吡罗和诺米芬辛）的注入。电极位置用离子电渗注入的染料滂胺天蓝进行标记。我们发现，EOF标记分子对乙酰氨基酚（AP）和2-（4-硝基苯氧基）乙醇对纹状体中电诱发的多巴胺释放或电极的敏感性没有影响。此外，我们证实，通过离子电渗疗法短暂（30秒）注入雷氯必利、喹吡罗或诺米芬辛足以影响自身受体调节和多巴胺的再摄取。这些效应的持续时间各不相同，表明该技术可用于研究受体动力学。
