Department of Pathology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
Cell Mol Life Sci. 2011 Jul;68(14):2481-92. doi: 10.1007/s00018-010-0572-7.
It has recently been demonstrated that not only mature B lymphocytes, but also non-lymphoid cells, including cancer cells and neurons, express IgG. In the eye, an important immune privileged site, the presence of IgG has been ascribed to IgG entering the eye through breaches of the blood–ocular barrier. Here we demonstrate that the eye itself can produce IgG intrinsically. Applying immunohistochemistry, in situ hybridization, and RT-PCR, several intraocular structures were found to express proteins and mRNA transcripts of IgG heavy chains, light chains, V(D)J rearrangements, and enzymes required for V(D)J recombination. IgG receptors were also detected in the intraocular epithelium and endothelium. The extensive distribution of IgG and its receptors in intraocular structures indicates that locally produced IgG could play a significant role in maintaining the ocular microenvironment and protection of the eyes, and it might also be involved in the pathogenesis of age-related macular degeneration and some inflammatory diseases.
最近已经证明，不仅成熟的 B 淋巴细胞，而且包括癌细胞和神经元在内的非淋巴细胞也表达 IgG。在眼睛这个重要的免疫特惠部位，IgG 的存在归因于 IgG 通过血-眼屏障的破裂进入眼睛。在这里，我们证明眼睛本身可以内在地产生 IgG。应用免疫组织化学、原位杂交和 RT-PCR，发现几个眼内结构表达 IgG 重链、轻链、V(D)J 重排以及 V(D)J 重组所需的酶的蛋白和 mRNA 转录本。IgG 受体也在眼内上皮和内皮中被检测到。IgG 及其受体在眼内结构中的广泛分布表明，局部产生的 IgG 可能在维持眼内微环境和保护眼睛方面发挥重要作用，它也可能与年龄相关性黄斑变性和一些炎症性疾病的发病机制有关。
