Gray D W
Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, England.
Surg Gynecol Obstet. 1990 Mar;170(3):225-32.
Aspects of islet transplantation in the primate have been investigated using a model of autotransplantation of islets in the monkey after total pancreatectomy. The results showed that the subcapsule of the kidney was not a good site for implantation of relatively impure islet preparations, or of purified islet preparations if the mass of tissue implanted was marginal. The spleen was a much better site for the islet implantation, allowing fasting normoglycemia in many of the animals. The results of histologic examination of the spleen four to six weeks after intrasplenic islet transplantation showed good preservation of islet morphologic features, with numerous islets scattered throughout the spleen. Cells secreting insulin, glucagon and somatostatin were present in approximately normal proportions. Transplantation of islets into the portal vein produced good short and long term function. A number of grafts failed between four and 18 months, this failure being related to the function demonstrated at six weeks by intravenous glucose tolerance tests. Animals with poor initial function tended to fail early. However, those with good initial function continued to gain weight and remain normoglycemic up to 2.5 years, providing encouragement to improve further the yield and purity of the islet preparation as well as seeking more efficient techniques for islet implantation.
利用全胰切除术后猴胰岛自体移植模型，对灵长类动物胰岛移植的各个方面进行了研究。结果表明，对于相对不纯的胰岛制剂，或植入组织量较少的纯化胰岛制剂而言，肾被膜不是一个理想的植入部位。脾脏是胰岛植入的更好部位，许多动物在此植入后可实现空腹血糖正常。胰岛脾内移植四至六周后脾脏的组织学检查结果显示，胰岛形态特征保存良好，脾脏内散布着大量胰岛。分泌胰岛素、胰高血糖素和生长抑素的细胞比例大致正常。胰岛门静脉移植产生了良好的短期和长期功能。一些移植物在4至18个月之间失败，这种失败与六周时静脉葡萄糖耐量试验所显示的功能有关。初始功能较差的动物往往早期失败。然而，那些初始功能良好的动物体重持续增加，血糖维持正常长达2.5年，这为进一步提高胰岛制剂的产量和纯度以及寻找更有效的胰岛植入技术提供了动力。
