Li Xiaohua, Park Nam Il, Xu Hui, Woo Sun-Hee, Park Cheol Ho, Park Sang Un
Department of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-754, Korea.
J Agric Food Chem. 2010 Dec 8;58(23):12176-81. doi: 10.1021/jf103310g. Epub 2010 Nov 9.
Common buckwheat (Fagopyrum esculentum) is a short-season grain crop that is a source of rutin and other phenolic compounds. In this study, we isolated the cDNAs of 11 F. esculentum enzymes in the flavonoid biosynthesis pathway, namely, phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL) 1 and 2, chalcone synthase (CHS), chalcone isomerase (CHI), flavone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS) 1 and 2, and anthocyanidin synthase (ANS). Quantitative real-time polymerase chain reaction analysis showed that these genes were most highly expressed in the stems and roots. However, high performance liquid chromatography analysis indicated that their flavonoid products, such as rutin and catechin, accumulated in the flowers and leaves. These results suggested that flavonoids may be transported within F. esculentum. In addition, light and dark growth conditions affected the expression levels of the biosynthesis genes and accumulation of phenolic compounds in F. esculentum sprouts.
普通荞麦（苦荞麦）是一种生长季短的谷类作物，是芦丁和其他酚类化合物的来源。在本研究中，我们分离了黄酮生物合成途径中11种苦荞麦酶的cDNA，即苯丙氨酸解氨酶（PAL）、肉桂酸4-羟化酶（C4H）、4-香豆酸：辅酶A连接酶（4CL）1和2、查尔酮合酶（CHS）、查尔酮异构酶（CHI）、黄酮3-羟化酶（F3H）、类黄酮3'-羟化酶（F3'H）、黄酮醇合酶（FLS）1和2以及花青素合酶（ANS）。实时定量聚合酶链反应分析表明，这些基因在茎和根中表达量最高。然而，高效液相色谱分析表明，它们的黄酮类产物，如芦丁和儿茶素，在花和叶中积累。这些结果表明，黄酮类化合物可能在苦荞麦体内运输。此外，光照和黑暗生长条件影响了苦荞麦芽中生物合成基因的表达水平和酚类化合物的积累。
