10 个多态微卫星标记在高原鼠兔（Ochotona curzoniae）亲权分析和谱系分析中的有效性。
Effectiveness of 10 polymorphic microsatellite markers for parentage and pedigree analysis in plateau pika (Ochotona curzoniae).
机构信息
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, the Chinese Academy of Sciences, 59 XiGuan Avenue, Xining, Qinghai 810001, PR China.
出版信息
BMC Genet. 2010 Nov 10;11:101. doi: 10.1186/1471-2156-11-101.
BACKGROUND
The plateau pika (Ochotona curzoniae) is an underground-dwelling mammal, native to the Tibetan plateau of China. A set of 10 polymorphic microsatellite loci has been developed earlier. Its reliability for parentage assignment has been tested in a plateau pika population. Two family groups with a known pedigree were used to validate the power of this set of markers.
RESULTS
The error in parentage assignment using a combination of these 10 loci was very low as indicated by their power of discrimination (0.803 - 0.932), power of exclusion (0.351 - 0.887), and an effectiveness of the combined probability of exclusion in parentage assignment of 99.999%.
CONCLUSION
All the offspring of a family could be assigned to their biological mother; and their father or relatives could also be identified. This set of markers therefore provides a powerful and efficient tool for parentage assignment and other population analyses in the plateau pika.
背景
高原鼠兔（Ochotona curzoniae）是一种穴居哺乳动物，原产于中国青藏高原。先前已经开发出了一套 10 个多态性微卫星位点。在高原鼠兔种群中已经测试了其用于亲子关系鉴定的可靠性。使用具有已知谱系的两个家系群体来验证这组标记的能力。
结果
使用这 10 个位点的组合进行亲子关系鉴定的错误率非常低，这表明其鉴别力（0.803-0.932）、排除力（0.351-0.887）和亲子关系鉴定中排除概率的综合有效性均很高，达到 99.999%。
结论
家系中的所有后代都可以被分配给其生母；其父亲或亲属也可以被鉴定出来。因此，这套标记物为高原鼠兔的亲子关系鉴定和其他种群分析提供了一种强大而有效的工具。
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