Hayashi S, Noguchi T
Department of Biochemistry, Kyushu Dental College, Kitakyushu, Japan.
Biochem Biophys Res Commun. 1990 Feb 14;166(3):1467-70. doi: 10.1016/0006-291x(90)91032-n.
The subcellular distribution of alanine: glyoxylate aminotransferase 1 in guinea pig and rabbit kidneys was examined by centrifugation in a sucrose density gradient. The enzyme was located in the peroxisomes of guinea pig kidney and cross-reactive with the antibody against rat liver alanine: glyoxylate aminotransferase 1. This is the first report on the presence of the enzyme in the peroxisomes of mammalian kidney. The enzyme was found to be located in the mitochondria but not in the peroxisomes in rabbit kidney.
通过在蔗糖密度梯度中离心,研究了豚鼠和兔肾脏中丙氨酸:乙醛酸转氨酶1的亚细胞分布。该酶位于豚鼠肾脏的过氧化物酶体中,与抗大鼠肝脏丙氨酸:乙醛酸转氨酶1的抗体发生交叉反应。这是关于该酶存在于哺乳动物肾脏过氧化物酶体中的首次报道。发现该酶位于兔肾脏的线粒体中,而不在过氧化物酶体中。