Aluclu Mehmet U, Arslan Seyfi, Acar Abdullah, Guzel Aslan, Bahceci Selen, Yaldiz Mehmet
Department of Neurology, School of Medicine, Dicle University, Diyarbakir, 21280, Turkey. Tel. +90 (412) 2488001. Ext. 4541. Fax. +90 (412) 2488440. E-mail:
Neurosciences (Riyadh). 2008 Apr;13(2):113-6.
To evaluate the effects of memantine on infarct size in cerebral ischemia and on neurological outcome after temporary middle cerebral artery occlusion (MCAO) and reperfusion in rats.
In this study, performed between 2002-2004 in Dicle University School of Medicine, Diyarbakir, Turkey, 30 adult Sprague-Dawley rats were used. Cerebral ischemia was constituted by the intraluminal filament method with a 4-0 nylon suture. Reperfusion was started after 2 hours of MCAO. The rats were randomly divided into 2 groups as control and memantine. Saline 0.9% (0.5 ml/kg) and memantine (30 mg/kg) were administered via nasogastric intubations. Three coronal slices of 2 mm thickness were obtained from cerebrum, cerebellum, and brain stem, and were stained with a 2% solution of triphenyltetrazolium chloride. Transparent sheets were placed over each section and the areas of the brain and infarct were measured.
Forty-five slices from each group (total 90) were obtained. Percent of ischemic area (%) in cerebrum, cerebellum, and brain stem level in memantine was lower than those of the control group (p<0.0001). In addition, we determined an improvement in neurological score at 24th and 72nd hours in the rats that have been given memantine. The memantine group showed significantly better recovery than the control group (p<0.0001).
We concluded that memantine may decrease ischemic area in experimental cerebral ischemia in rats and it seems that memantine may be beneficial in cerebral ischemia.
评估美金刚对大鼠大脑中动脉短暂闭塞（MCAO）及再灌注后脑缺血梗死面积和神经功能结局的影响。
本研究于2002年至2004年在土耳其迪亚巴克尔的迪克莱大学医学院进行，使用了30只成年Sprague-Dawley大鼠。采用4-0尼龙缝线经腔内插入法造成脑缺血。MCAO 2小时后开始再灌注。大鼠被随机分为对照组和美金刚组。通过鼻胃管分别给予0.9%生理盐水（0.5 ml/kg）和美金刚（30 mg/kg）。从大脑、小脑和脑干获取3片厚度为2 mm的冠状切片，并用2%的氯化三苯基四氮唑溶液染色。在每片切片上放置透明片，测量脑和梗死灶的面积。
每组获得45片切片（共90片）。美金刚组大脑、小脑和脑干水平的缺血面积百分比（%）低于对照组（p<0.0001）。此外，我们发现给予美金刚的大鼠在第24小时和第72小时神经评分有所改善。美金刚组的恢复情况明显优于对照组（p<0.0001）。
我们得出结论，美金刚可能会减少大鼠实验性脑缺血的缺血面积，并且美金刚似乎对脑缺血有益。
