Aluclu Mehmet Ufuk, Acar Abdullah, Guzel Aslan, Bahceci Selen, Yaldiz Mehmet
Department of Neurology, School of Medicine, Dicle University, Diyarbakir, Turkey.
Neuro Endocrinol Lett. 2007 Apr;28(2):170-4.
Majority of severe disabilities in adults are caused by stroke. The aim of our study is to learn the effects of erythropoietin (EPO), on infarct size in cerebral ischemia and to determine neurological behavioral scores and histopathological evaluation.
MATERIAL & METHODS: In this study 30 adult Sprague-Dawney rats were used. Cerebral ischemia was constituted by intraluminal filament method with a 4-0-nylon suture. Reperfusion was started after two hours of middle cerebral artery occlusion. The rats were randomly divided into two groups as follow: control and EPO groups. Saline 0.9% (0.5 ml/kg) and EPO (5 000 U/kg) was administered intraperitoneally in the groups. Three coronal slices in two millimeters thickness were obtained from cerebrum, cerebellum and brain stem, and were stained with a 2% solution of triphenyltetrazolium chloride. Transparent sheets were placed over each section and the areas of the brain and infarct were measured. The neurological scores were determined at 24th, 48th and 72nd hours after reperfusion.
Percent of ischemic area (%) in cerebrum, cerebellum and brain stem level in EPO groups were less than those of control group (p<0.0001). In addition, we determined that EPO group was better than controls of neurologic score and histopathologically after cerebral ischemia.
We concluded that EPO may decrease ischemic area in experimental cerebral ischemia in rats and it seems that EPO may be beneficial.
成年人中的大多数严重残疾是由中风引起的。我们研究的目的是了解促红细胞生成素（EPO）对脑缺血梗死面积的影响，并确定神经行为评分和组织病理学评估结果。
本研究使用了30只成年Sprague-Dawney大鼠。采用4-0尼龙缝线经腔内插入法造成脑缺血。大脑中动脉闭塞两小时后开始再灌注。大鼠被随机分为两组：对照组和EPO组。分别向两组大鼠腹腔注射0.9%生理盐水（0.5 ml/kg）和EPO（5000 U/kg）。从大脑、小脑和脑干获取厚度为2毫米的三个冠状切片，并用2%的氯化三苯基四氮唑溶液染色。在每个切片上放置透明片，测量脑和梗死灶的面积。在再灌注后第24、48和72小时测定神经学评分。
EPO组大脑、小脑和脑干水平的缺血面积百分比（%）低于对照组（p<0.0001）。此外，我们确定EPO组在脑缺血后的神经学评分和组织病理学方面均优于对照组。
我们得出结论，EPO可能会减少大鼠实验性脑缺血中的缺血面积，并且似乎EPO可能是有益的。
