N-乙酰半乳糖胺基转移酶的一种内源性抑制剂可抑制培养中的视网膜神经元分化。

An endogenous inhibitor of N-acetylgalactosaminyltransferase inhibits retina neuron differentiation in culture.

作者信息

Quiroga S, Panzetta P, Caputto R

机构信息

Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.

出版信息

Brain Res. 1990 Feb 5;508(2):337-40. doi: 10.1016/0006-8993(90)90420-g.

DOI:10.1016/0006-8993(90)90420-g

PMID:2106364

Abstract

An inhibitor of N-acetylgalactosamine:GM3, N-acetylgalactosaminyltransferase (EC 2.4.1.92) from chicken blood serum, was tested for its activity on embryonic chicken neural retina in culture. The inhibitor did not change the cellular protein content of the cultures but produced a significant reduction of the labeling of gangliosides. The ratio of labeling of GD3 to GD1a increased from about 0.1 to about 0.8 in the cells cultured without or with the inhibitor, respectively. A striking effect of the inhibitor was seen on the morphology of the neurons, those cultured in its presence being practically devoid of neurites. Glial flat cells were apparently not affected.

摘要

对从鸡血清中提取的N-乙酰半乳糖胺：GM3 N-乙酰半乳糖胺基转移酶（EC 2.4.1.92）抑制剂，在体外培养的鸡胚神经视网膜上进行了活性测试。该抑制剂未改变培养物中的细胞蛋白质含量，但导致神经节苷脂标记显著减少。在分别添加或未添加抑制剂的培养细胞中，GD3与GD1a的标记比率从约0.1增加到约0.8。该抑制剂对神经元形态有显著影响，在其存在下培养的神经元几乎没有神经突。胶质扁平细胞显然未受影响。