水飞蓟素对白化大鼠乙醇诱导的肝毒性中血清谷丙转氨酶和谷氨酰转肽酶水平的影响。
Affect of silymarin on serum levels of ALT and GGT in ethanol induced hepatotoxicity in albino rats.
作者信息
Habib-ur-Rehman Muhammad, Mahmood Tariq, Salim Taher, Afzal Naeema, Ali Nasir, Iqbal Javed, Tahir Muhammad, Khan Asif
机构信息
Department of Anatomy, Nawaz Sharif Medical College, University of Gujrat, Pakistan.
出版信息
J Ayub Med Coll Abbottabad. 2009 Oct-Dec;21(4):73-5.
BACKGROUND
Alcoholic liver disease is a worldwide health problem. At least 80% of heavy drinkers have been reported to develop steatosis, 10-35% alcoholic hepatitis, and approximately 10% liver cirrhosis. The objective of this study was to determine the affect of silymarin on the levels of serum ALT and GGT in ethanol induced hepatotoxicity in albino rats. This study was an experimental Randomised Control Trial (RCT), and was conducted at the experimental research laboratory of University of Health Sciences, Lahore, from January 2007 to December 2007.
METHODS
Eighteen male albino rats of 6-8 weeks age, weighing 150-200 gm each were divided into 3 groups of 6 rats each. Group A served as control, Group B was given ethanol at a dose of 0.6 ml (0.5 gm)/100 gm/day and group C was given ethanol and silymarin at a dose of 0.5 gm/100 gm/day, and 20 mg/100 gm/day respectively for 8 weeks. At the end of the experiment, each animal was euthenised with chloroform. Blood was drawn from each animal by cardiac puncture for liver function tests (ALT and GGT). After taking blood sample, each euthenised animal was sacrificed and then its liver was removed for gross and histological examination.
RESULTS
The mean values of serum alanine-aminotransferase (ALT) in groups A, B and C were 28.16 +/- 7.13, 82.33 +/- 10.89 and 49.66 +/- 6.12 U/L respectively, whereas, the mean values of gamma-glutamyl transferase (GGT) in groups A, B and C were 27.33 +/- 3.05, 79.33 +/- 4.37 and 45.66 +/- 1.85 U/L respectively. ANOVA showed significant (p < 0.05) difference in mean value of these serum enzymes among groups. Post Hoc test, using the Tukey honestly significant difference (HSD) showed that there was significant (p < 0.05) increase in mean value of ALT and GGT in group B as compared to group A and C. This test also showed that there was significant (p < 0.05) decrease in mean value of these enzymes in-group C as compared to group B.
CONCLUSION
Silymarin tends to normalise liver function test in alcoholic liver disease.
背景
酒精性肝病是一个全球性的健康问题。据报道，至少80%的重度饮酒者会出现脂肪变性，10 - 35%会患酒精性肝炎，约10%会患肝硬化。本研究的目的是确定水飞蓟宾对白化大鼠乙醇诱导的肝毒性中血清谷丙转氨酶（ALT）和谷氨酰转肽酶（GGT）水平的影响。本研究是一项实验性随机对照试验（RCT），于2007年1月至2007年12月在拉合尔健康科学大学的实验研究实验室进行。
方法
18只6 - 8周龄的雄性白化大鼠，每只体重150 - 200克，分为3组，每组6只。A组作为对照组，B组给予剂量为0.6毫升（0.5克）/100克/天的乙醇，C组给予剂量分别为0.5克/100克/天的乙醇和20毫克/100克/天的水飞蓟宾，持续8周。实验结束时，每只动物用氯仿安乐死。通过心脏穿刺从每只动物采集血液进行肝功能测试（ALT和GGT）。采集血样后，处死每只安乐死的动物，然后取出其肝脏进行大体和组织学检查。
结果
A组、B组和C组血清丙氨酸转氨酶（ALT）的平均值分别为28.16±7.13、82.33±10.89和49.66±6.12 U/L，而A组、B组和C组γ-谷氨酰转肽酶（GGT）的平均值分别为27.33±3.05、79.33±4.37和45.66±1.85 U/L。方差分析显示这些血清酶的平均值在各组之间存在显著差异（p < 0.05）。使用Tukey真实显著差异（HSD）的事后检验表明，与A组和C组相比，B组ALT和GGT的平均值有显著升高（p < 0.05）。该检验还表明，与B组相比，C组这些酶的平均值有显著降低（p < 0.05）。
结论
水飞蓟宾倾向于使酒精性肝病患者的肝功能测试恢复正常。
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