水飞蓟素对N-亚硝基二乙胺诱导大鼠肝癌发生的影响。
Effect of silymarin on N-nitrosodiethylamine induced hepatocarcinogenesis in rats.
作者信息
Ramakrishnan G, Augustine T A, Jagan S, Vinodhkumar R, Devaki T
机构信息
Department of Biochemistry, University of Madras, Tamilnadu, India.
出版信息
Exp Oncol. 2007 Mar;29(1):39-44.
AIM
To study the effect of silymarin on the levels of tumor markers and MDA (malondialdehyde)-DNA adduct formation during N-nitrosodiethylamine induced hepatocellular carcinoma in male Wistar albino rats.
METHODS
The levels of AFP, CEA and activities of liver marker enzymes in serum, MDA-DNA immunohistochemistry were done according to standard procedures in the control and experimental groups of rats.
RESULTS
Hepatocellular carcinoma was evidenced from significant (p < 0.05) increases of alpha-fetoprotein, carcinoembryonic antigen, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, gamma-glutamyltransferase and 5'-nucleotidase in serum and increased MDA-DNA adducts were also observed in the tissue sections of hepatocellular carcinoma. Silymarin treatment significantly attenuated the alteration of these markers and decreased the levels of MDA-DNA adduct formation.
CONCLUSION
Silymarin could be developed as a promising chemotherapeutic adjuvant for the treatment of liver cancer.
目的
研究水飞蓟素对雄性Wistar白化大鼠在N-亚硝基二乙胺诱导的肝细胞癌过程中肿瘤标志物水平及丙二醛（MDA）-DNA加合物形成的影响。
方法
按照标准程序对大鼠对照组和实验组进行血清中甲胎蛋白（AFP）、癌胚抗原（CEA）水平及肝脏标志物酶活性检测，以及MDA-DNA免疫组化检测。
结果
血清中甲胎蛋白、癌胚抗原、天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、酸性磷酸酶、乳酸脱氢酶、γ-谷氨酰转移酶和5'-核苷酸酶显著升高（p < 0.05），证实发生了肝细胞癌，并且在肝细胞癌组织切片中也观察到MDA-DNA加合物增加。水飞蓟素治疗显著减轻了这些标志物的改变，并降低了MDA-DNA加合物形成水平。
结论
水飞蓟素有望开发成为一种治疗肝癌的有前景的化疗辅助药物。
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