Broad Institute of MIT & Harvard, Cambridge, MA 02142, USA.
Bioinformatics. 2011 Jan 15;27(2):266-7. doi: 10.1093/bioinformatics/btq624. Epub 2010 Nov 9.
PriSM is a set of algorithms designed to select and match degenerate primer pairs for the amplification of viral genomes. The design of panels of hundreds of primer pairs takes just hours using this program, compared with days using a manual approach. PriSM allows for rapid in silico optimization of primers for downstream applications such as sequencing. As a validation, PriSM was used to create an amplification primer panel for human immunodeficiency virus (HIV) Clade B.
The program is freely available for use at: www.broadinstitute.org/perl/seq/specialprojects/primerDesign.cgi.
PriSM 是一组旨在选择和匹配简并引物对以扩增病毒基因组的算法。使用该程序设计数百对引物的面板仅需数小时,而使用手动方法则需要数天。PriSM 允许快速对下游应用(如测序)的引物进行计算机优化。作为验证,PriSM 用于创建人类免疫缺陷病毒(HIV)B 型的扩增引物面板。
该程序可免费在以下网址使用:www.broadinstitute.org/perl/seq/specialprojects/primerDesign.cgi。