Boutros Paul C, Okey Allan B
Department of Pharmacology, University of Toronto, Medical Sciences Building, 1 King's College Circle, Toronto, Ontario, M5S-1A8 Canada.
Bioinformatics. 2004 Oct 12;20(15):2399-400. doi: 10.1093/bioinformatics/bth257. Epub 2004 Apr 8.
We developed a CGI/Perl-based web server to perform in silico polymerase chain reaction (PCR) on PCR primer sequences. The PUNS (Primer-UniGene Selectivity) server simulates PCR reactions by running BLASTN analysis on user-entered primer pairs against both the transcriptome and the genome to assess primer specificity. PUNS is particularly suited for the identification of highly selective primers for quantitative microarray validation.
Both system access and source-code are freely available at http://okeylabimac.med.utoronto.ca/PUNS.
我们开发了一个基于CGI/Perl的网络服务器，用于对PCR引物序列进行计算机模拟聚合酶链反应（PCR）。PUNS（引物-单基因选择性）服务器通过针对转录组和基因组对用户输入的引物对运行BLASTN分析来模拟PCR反应，以评估引物特异性。PUNS特别适用于鉴定用于定量微阵列验证的高选择性引物。
系统访问和源代码均可在http://okeylabimac.med.utoronto.ca/PUNS免费获取。
