Khomutov G, Fry I V, Huflejt M E, Packer L
Membrane Bioenergetics Group, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Arch Biochem Biophys. 1990 Mar;277(2):263-7. doi: 10.1016/0003-9861(90)90577-l.
The effect of adaptation to saline growth of a fresh water cyanobacterium Synechococcus 6311 on components of the cytoplasmic membranes and thylakoids was investigated. Significant changes in membrane surface charge, lipid, fatty acid, and carotenoid composition were observed upon transfer of the cells from a low salt (0.015 M NaCl) to a high salt (0.50 M NaCl) growth medium. Very similar changes in the polar lipid classes and fatty acid composition were observed in both membranes, but changes in fluidity and surface charge and a significant shift in the protein to lipid ratio were only apparent in the cytoplasmic membranes. The fluidity and surface charge data correlate well with functional studies and we can attribute the cytoplasmic membrane as the major site of interaction and adaptation to the saline environment.
研究了淡水蓝藻聚球藻6311适应盐生长对细胞质膜和类囊体成分的影响。当细胞从低盐（0.015 M NaCl）生长培养基转移到高盐（0.50 M NaCl）生长培养基时，观察到膜表面电荷、脂质、脂肪酸和类胡萝卜素组成发生了显著变化。在两种膜中都观察到极性脂质类别和脂肪酸组成非常相似的变化，但流动性和表面电荷的变化以及蛋白质与脂质比率的显著变化仅在细胞质膜中明显。流动性和表面电荷数据与功能研究很好地相关，我们可以将细胞质膜归因于与盐环境相互作用和适应的主要部位。
