College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China.
College of Biotechnology, Xinjiang Agricultural Vocational and Technical University, Changji 831100, China.
Genes (Basel). 2024 Sep 7;15(9):1176. doi: 10.3390/genes15091176.
The problem of soil salinization has seriously hindered agricultural development. Cotton is a pioneering salinity-tolerant crop, so harvesting its key salinity-tolerant genes is important for improving crop salt tolerance. In this study, we analyzed changes in the transcriptome expression profiles of the salt-tolerant cultivar Lu Mian 28 (LM) and the salt-sensitive cultivar Zhong Mian Suo 12 (ZMS) after applying salt stress, and we constructed weighted gene co-expression networks (WGCNA). The results indicated that photosynthesis, amino acid biosynthesis, membrane lipid remodeling, autophagy, and ROS scavenging are key pathways in the salt stress response. Plant-pathogen interactions, plant hormone signal transduction, the mitogen-activated protein kinase (MAPK) signaling pathway, and carotenoid biosynthesis are the regulatory networks associated with these metabolic pathways that confer cotton salt tolerance. The gene-weighted co-expression network was used to screen four modules closely related to traits, identifying 114 transcription factors, including WRKYs, ERFs, NACs, bHLHs, bZIPs, and MYBs, and 11 hub genes. This study provides a reference for acquiring salt-tolerant cotton and abundant genetic resources for molecular breeding.
土壤盐渍化问题严重阻碍了农业发展。棉花是一种具有开拓性耐盐性的作物，因此，收获其关键的耐盐性基因对于提高作物耐盐性非常重要。在这项研究中，我们分析了耐盐品种鲁棉 28（LM）和盐敏感品种中棉所 12（ZMS）在施加盐胁迫后转录组表达谱的变化，并构建了加权基因共表达网络（WGCNA）。结果表明，光合作用、氨基酸生物合成、膜脂重塑、自噬和 ROS 清除是盐胁迫反应的关键途径。植物-病原体相互作用、植物激素信号转导、丝裂原激活蛋白激酶（MAPK）信号通路和类胡萝卜素生物合成是与这些代谢途径相关的调节网络，赋予棉花耐盐性。基因加权共表达网络用于筛选与性状密切相关的四个模块，鉴定出 114 个转录因子，包括 WRKYs、ERFs、NACs、bHLHs、bZIPs 和 MYBs，以及 11 个枢纽基因。本研究为获得耐盐棉花和丰富的分子育种遗传资源提供了参考。
