循环的人 CD4 和 CD8 T 细胞内没有大量的 CCR5 细胞内池。
Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5.
机构信息
Department of Medicine, Division of Infectious Diseases, Case Western Reserve University/University Hospitals/Case Medical Center, Cleveland, OH, USA.
出版信息
Blood. 2011 Jul 28;118(4):1015-9. doi: 10.1182/blood-2010-05-282509. Epub 2010 Nov 10.
Abstract
CC Chemokine Receptor 5 (CCR5) is an important mediator of chemotaxis and the primary coreceptor for HIV-1. A recent report by other researchers suggested that primary T cells harbor pools of intracellular CCR5. With the use of a series of complementary techniques to measure CCR5 expression (antibody labeling, Western blot, quantitative reverse transcription polymerase chain reaction), we established that intracellular pools of CCR5 do not exist and that the results obtained by the other researchers were false-positives that arose because of the generation of irrelevant binding sites for anti-CCR5 antibodies during fixation and permeabilization of cells.
摘要
细胞表面趋化因子受体 5（CCR5）是趋化作用的重要介质，也是 HIV-1 的主要核心受体。最近其他研究人员的一份报告表明，原代 T 细胞中存在细胞内 CCR5 池。我们使用一系列互补技术来测量 CCR5 的表达（抗体标记、Western blot、定量逆转录聚合酶链反应），结果表明细胞内 CCR5 池并不存在，而其他研究人员的结果是假阳性，这是由于在固定和透化细胞过程中产生了与抗 CCR5 抗体无关的结合位点。
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