Departamento de Microbiologia, Universidade Federal de Viçosa,, Viçosa, Minas Gerais, Brazil.
Metallomics. 2010 Feb;2(2):162-6. doi: 10.1039/b915780h. Epub 2010 Jan 7.
The in vivo bioavailability of Se was investigated in enriched Pleurotus ostreatus mushrooms. A bioavailability study was performed using 64 Wistar male rats separated in 8 groups and fed with different diets: without Se, with mushrooms without Se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg(-1) Se and a normal diet containing 0.15 mg kg(-1) of Se using sodium selenate. The experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the Association of Official Analytical Chemists. After five weeks, the rats were sacrificed under carbon dioxide, and blood was drawn by heart puncture. Blood plasma was separated by centrifugation. The total Se concentration in the plasma of rats fed with enriched mushrooms was higher than in rats fed with a normal diet containing sodium selenate. The plasma protein profiles were obtained using size exclusion chromatography (SEC) and UV detectors. Aliquots of effluents (0.5 mL per minute) were collected throughout in the end of the chromatographic column. However, Se was determined by graphite furnace atomic absorption spectrometry (GF AAS) only in the aliquots where proteins were detected by SEC-UV. The plasma protein profile of rats fed with different diets was similar. The highest Se concentration was observed in a peptide presenting 8 kDa. Furthermore, the higher Se concentration in this peptide was obtained for rats fed with a diet using enriched mushrooms (7 μg L(-1) Se) compared to other diets (2-5 μg L(-1) Se). These results showed that Se-enriched mushrooms can be considered as an alternative Se food source for humans, due to their high bioavailability.
采用 64 只雄性 Wistar 大鼠进行了生物有效性研究，将其分为 8 组，分别投喂不同的饲料：无硒、无硒蘑菇、含 0.15、0.30 或 0.45mg/kg 硒的富硒蘑菇和用亚硒酸钠作为硒源的正常饲料（含 0.15mg/kg 硒）。实验分为两个阶段：耗竭（14 天）和补充（21 天），根据美国分析化学家协会的方法进行。五周后，大鼠在二氧化碳中被处死，通过心脏穿刺采血。通过离心分离血浆。与投喂正常含硒酸钠饲料的大鼠相比，投喂富硒蘑菇的大鼠血浆中的总硒浓度更高。采用体积排阻色谱（SEC）和紫外检测器获得大鼠血浆的蛋白质图谱。整个色谱柱末端以 0.5mL/min 的速度收集洗脱液。然而，只有在用 SEC-UV 检测到蛋白质的洗脱液中才用石墨炉原子吸收光谱法（GF AAS）测定硒。投喂不同饲料的大鼠的血浆蛋白质图谱相似。在分子量为 8kDa 的肽中观察到最高的硒浓度。此外，在投喂富硒蘑菇饲料的大鼠中，该肽中的硒浓度最高（7μg/L 硒），而其他饲料（2-5μg/L 硒）中则较低。这些结果表明，富硒蘑菇可以作为人类的硒源，因为其生物有效性高。
