Universidade Federal de São Paulo, Departamento de Química, Diadema, 09972-270, Brazil.
Universidade Federal do ABC, Centro de Ciências Naturais e Humanas, Santo André, 09210-580, Brazil.
Sci Rep. 2019 Jul 25;9(1):10827. doi: 10.1038/s41598-019-47338-7.
Mushrooms are bioaccumulating organisms commonly used in selenium (Se) enrichment studies. However, the addition of Se in the culture medium may alter the distribution of other essential elements in the mushroom fruiting body. To evaluate the effects of the Se enrichment, Ca, Mg, and K distributions in pink oyster (Pleurotus djamor) and K and Mg distributions in white oyster (Pleurotus ostreatus) mushrooms were mapped by laser-induced breakdown spectroscopy (LIBS), which can be used at room temperature and requires minimal or no sample preparation. It was verified that Se enrichment favoured the accumulation of Ca in the lower part of the pink oyster mushroom and prevented the transport of this element to the edges and tops. The Se enrichment also altered the distribution of K and Mg, decreasing the numerical correlation between the K and Mg distributions (R² = 0.5871). In the white oyster mushroom, however, despite the changes in the morphological characteristics of the fruiting bodies after enrichment, there were generally nonsignificant differences in the K and Mg distributions between the control and the Se-enriched mushrooms.
蘑菇是生物蓄积性生物体，常用于硒（Se）富集研究。然而，培养基中添加 Se 可能会改变蘑菇子实体中其他必需元素的分布。为了评估 Se 富集的影响，通过激光诱导击穿光谱（LIBS）对粉红牡蛎（Pleurotus djamor）中的 Ca、Mg 和 K 分布以及白牡蛎（Pleurotus ostreatus）中的 K 和 Mg 分布进行了映射，LIBS 可在室温下使用，且几乎不需要或无需样品制备。结果表明，Se 富集有利于 Ca 在粉红牡蛎蘑菇下部的积累，并阻止该元素向边缘和顶部运输。Se 富集还改变了 K 和 Mg 的分布，降低了 K 和 Mg 分布之间的数值相关性（R²=0.5871）。然而，在白牡蛎蘑菇中，尽管富硒后子实体的形态特征发生了变化，但富硒蘑菇和对照蘑菇之间的 K 和 Mg 分布通常没有显著差异。
