Institute of Zoonoses, College of Animal Sciences and Veterinary Medicine, Jilin University, Changchun, China.
J Appl Microbiol. 2011 Jan;110(1):333-40. doi: 10.1111/j.1365-2672.2010.04888.x. Epub 2010 Nov 10.
To determine the antimicrobial activity of costus (Saussurea lappa) oil against Staphylococcus aureus, and to evaluate the influence of subinhibitory concentrations of costus oil on virulence-related exoprotein production in staph. aureus.
Minimal inhibitory concentrations (MICs) were determined using a broth microdilution method, and the MICs of costus oil against 32 Staph. aureus strains ranged from 0.15 to 0.6 μl ml(-1) . The MIC(50) and MIC(90) were 0.3 and 0.6 μl ml(-1) , respectively. Western blot, haemolytic, tumour necrosis factor (TNF) release and real-time RT-PCR assays were performed to evaluate the effects of subinhibitory concentrations of costus oil on virulence-associated exoprotein production in Staph. aureus. The data presented here show that costus oil dose dependently decreased the production of α-toxin, toxic shock syndrome toxin 1 (TSST-1) and enterotoxins A and B in both methicillin-sensitive Staph. aureus (MSSA) and methicillin-resistant Staph. aureus (MRSA).
Costus oil has potent antimicrobial activity against Staph. aureus, and the production of α-toxin, TSST-1 and enterotoxins A and B in Staph. aureus was decreased by costus oil.
The data suggest that costus oil may deserve further investigation for its potential therapeutic value in treating Staph. aureus infections. Furthermore, costus oil could be rationally applied in food products as a novel food preservative both to inhibit the growth of Staph. aureus and to repress the production of exotoxins, particularly staphylococcal enterotoxins.
测定菖蒲油对金黄色葡萄球菌的抗菌活性，并评估菖蒲油亚抑菌浓度对金黄色葡萄球菌毒力相关外蛋白产生的影响。
采用肉汤微量稀释法测定最小抑菌浓度（MIC），菖蒲油对 32 株金黄色葡萄球菌的 MIC 范围为 0.15-0.6μl/ml。MIC（50）和 MIC（90）分别为 0.3 和 0.6μl/ml。采用 Western blot、溶血、肿瘤坏死因子（TNF）释放和实时 RT-PCR 试验评估菖蒲油亚抑菌浓度对金黄色葡萄球菌毒力相关外蛋白产生的影响。结果显示，菖蒲油剂量依赖性地降低了甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）中α-毒素、中毒性休克综合征毒素 1（TSST-1）和肠毒素 A、B 的产生。
菖蒲油对金黄色葡萄球菌具有较强的抗菌活性，菖蒲油降低了金黄色葡萄球菌中α-毒素、TSST-1 和肠毒素 A、B 的产生。
这些数据表明，菖蒲油可能因其在治疗金黄色葡萄球菌感染方面的潜在治疗价值而值得进一步研究。此外，菖蒲油可作为一种新型食品防腐剂在食品中合理应用，既能抑制金黄色葡萄球菌的生长，又能抑制外毒素，特别是葡萄球菌肠毒素的产生。
