Department of Biology Education, Seoul National University, Seoul, Republic of Korea.
FEBS Lett. 2010 Dec 1;584(23):4731-4. doi: 10.1016/j.febslet.2010.11.003. Epub 2010 Nov 9.
PER3 is a member of the PERIOD genes, but does not play essential roles in the circadian clock. Depletion of Per3 by siRNA almost completely abolished activation of checkpoint kinase 2 (Chk2) after inducing DNA damage in human cells. In addition, Per3 physically interacted with ATM and Chk2. Per3 overexpression induced Chk2 activation in the absence of exogenous DNA damage, and this activation depended on ATM. Per3 overexpression also led to the inhibition of cell proliferation and apoptotic cell death. These combined results suggest that Per3 is a checkpoint protein that plays important roles in checkpoint activation, cell proliferation and apoptosis.
PER3 是 PERIOD 基因家族的一员，但在生物钟中不起关键作用。用 siRNA 耗尽 Per3 后，人细胞在诱导 DNA 损伤后几乎完全消除了检查点激酶 2 (Chk2) 的激活。此外，Per3 与 ATM 和 Chk2 发生物理相互作用。在没有外源性 DNA 损伤的情况下，过表达 Per3 诱导 Chk2 激活，这种激活依赖于 ATM。过表达 Per3 还导致细胞增殖和凋亡细胞死亡的抑制。这些综合结果表明，Per3 是一种检查点蛋白，在检查点激活、细胞增殖和凋亡中发挥重要作用。
