Becker R S, Suter M, Knight K L
Department of Microbiology, Loyola University of Chicago, Stritch School of Medicine, Maywood, IL 60153.
Eur J Immunol. 1990 Feb;20(2):397-402. doi: 10.1002/eji.1830200224.
Polyclonal B cell leukemias have been generated in 17- to 20-day old Emu-myc transgenic rabbits. To analyze the repertoire of VH genes utilized in early B cells, eight VDJ genes were cloned from these leukemic cells. The nucleotide sequences of these genes indicated that seven of the eight VDJ genes encoded prototype VHa1, VHa2 or VHa3 allotypes. The two VDJ genes encoding VHa1 molecules had VH segments with identical nucleotide sequences; similarly, the VH segments of the four VDJ genes encoding VHa2 molecules were identical, with the exception of a single base pair. These data suggest that a limited repertoire of VH genes were utilized in the generation of these VDJ genes. The DH segments of these genes were limited to two DH families, D1 and D2, indicating that a restricted repertoire of DH genes also had been utilized. Since these leukemic cells probably developed early in ontogeny, we suggest that this restricted utilization of VH and DH genes is representative of B cells from developmentally immature rabbits.
在17至20日龄的鸸鹋 - myc转基因兔中产生了多克隆B细胞白血病。为了分析早期B细胞中使用的VH基因库，从这些白血病细胞中克隆了8个VDJ基因。这些基因的核苷酸序列表明，8个VDJ基因中的7个编码原型VHa1、VHa2或VHa3同种异型。编码VHa1分子的两个VDJ基因具有核苷酸序列相同的VH区段；同样，编码VHa2分子的4个VDJ基因的VH区段除了一个碱基对外是相同的。这些数据表明，在这些VDJ基因的产生过程中使用了有限的VH基因库。这些基因的DH区段仅限于两个DH家族，即D1和D2，这表明也使用了有限的DH基因库。由于这些白血病细胞可能在个体发育早期发育，我们认为VH和DH基因的这种有限使用代表了发育不成熟兔子的B细胞。
