Savioz A, Jeenes D J, Kocher H P, Haas D
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Gene. 1990 Jan 31;86(1):107-11. doi: 10.1016/0378-1119(90)90121-7.
In a comparative study of housekeeping genes of Pseudomonas aeruginosa and Escherichia coli, the nucleotide sequence of a proline biosynthetic gene, proC, of P. aeruginosa has been determined. The subunit molecular mass (approximately 29 kDa) and the N-terminal amino acid sequence of purified delta 1-pyrroline 5-carboxylate reductase, the proC gene product, were in agreement with the proC nucleotide sequence. A survey of pairs of isofunctional genes from P. aeruginosa and E. coli reveals that within each pair, translated genes (including proC) have diverged more strongly than have untranslated genes specifying ribosomal or transfer RNAs. The translated genes, but not the untranslated ones, have a G + C content that is typical of the respective genomic G + C contents.
在一项对铜绿假单胞菌和大肠杆菌管家基因的比较研究中，已确定了铜绿假单胞菌脯氨酸生物合成基因proC的核苷酸序列。纯化的δ1-吡咯啉5-羧酸还原酶（proC基因产物）的亚基分子量（约29 kDa）和N端氨基酸序列与proC核苷酸序列一致。对铜绿假单胞菌和大肠杆菌的同功能基因对进行的一项调查显示，在每一对中，已翻译的基因（包括proC）比指定核糖体RNA或转移RNA的未翻译基因分歧更大。已翻译的基因，而非未翻译的基因，具有各自基因组典型的G + C含量。
