鼠伤寒沙门氏菌proC基因的转录调控
Transcriptional regulation of the proC gene of Salmonella typhimurium.
作者信息
Brady R A, Csonka L N
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
出版信息
J Bacteriol. 1988 May;170(5):2379-82. doi: 10.1128/jb.170.5.2379-2382.1988.
Abstract
We found that the expression of beta-galactosidase in Salmonella typhimurium strains carrying proC-lacZ fusions was neither repressed by excess proline nor derepressed by proline limitation. Except for a three- to fourfold decrease in the beta-galactosidase specific activity under conditions causing a severely reduced growth rate, the expression of the proC-lacZ fusions was nearly invariant under a variety of culture conditions. Thus, the proC gene is unlike most other amino acid biosynthetic genes in that its expression is nearly constitutive.
摘要
我们发现，携带proC-lacZ融合基因的鼠伤寒沙门氏菌菌株中β-半乳糖苷酶的表达既不会因脯氨酸过量而受到抑制，也不会因脯氨酸限制而解除抑制。除了在导致生长速率严重降低的条件下β-半乳糖苷酶比活性下降三到四倍外，proC-lacZ融合基因的表达在各种培养条件下几乎保持不变。因此，proC基因与大多数其他氨基酸生物合成基因不同，其表达几乎是组成型的。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[Reversible specific concentration of amino acids in Escherichia coli].[大肠杆菌中氨基酸的可逆特异性浓度]
Ann Inst Pasteur (Paris). 1956 Nov;91(5):693-720.
3
A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength.鼠伤寒沙门氏菌中的第三种L-脯氨酸通透酶，其在高渗透压强度的培养基中发挥作用。
J Bacteriol. 1982 Sep;151(3):1433-43. doi: 10.1128/jb.151.3.1433-1443.1982.
4
Conditionally transposition-defective derivative of Mu d1(Amp Lac).Mu d1（Amp Lac）的条件性转座缺陷衍生物
J Bacteriol. 1984 Jul;159(1):130-7. doi: 10.1128/jb.159.1.130-137.1984.
5
Genetic analysis of the proBA genes of Salmonella typhimurium: physical and genetic analyses of the cloned proB+ A+ genes of Escherichia coli and of a mutant allele that confers proline overproduction and enhanced osmotolerance.鼠伤寒沙门氏菌proBA基因的遗传分析：大肠杆菌克隆的proB⁺A⁺基因及赋予脯氨酸过量产生和增强渗透压耐受性的突变等位基因的物理和遗传分析。
J Bacteriol. 1983 Dec;156(3):1249-62. doi: 10.1128/jb.156.3.1249-1262.1983.
6
Linkage map of Salmonella typhimurium, Edition VI.鼠伤寒沙门氏菌连锁图谱，第六版。
Microbiol Rev. 1983 Sep;47(3):410-53. doi: 10.1128/mr.47.3.410-453.1983.
7
Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification.大肠杆菌δ1-吡咯啉-5-羧酸还原酶：基因序列、蛋白质过量表达及纯化
Nucleic Acids Res. 1982 Dec 11;10(23):7701-14. doi: 10.1093/nar/10.23.7701.
8
The gene-enzyme relationships of proline biosynthesis in Escherichia coli.大肠杆菌中脯氨酸生物合成的基因-酶关系
J Gen Microbiol. 1980 Jun;118(2):287-93. doi: 10.1099/00221287-118-2-287.
9
New spectrophotometric method for the determination of proline in tissue hydrolyzates.测定组织水解产物中脯氨酸的新分光光度法。
Anal Chem. 1970 Jun;42(7):702-6. doi: 10.1021/ac60289a036.
10
[Proline production regulation in E. coli K 12].[大肠杆菌K12中脯氨酸的生产调控]
Ann Inst Pasteur (Paris). 1971 Feb;120(2):126-43.
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