Department of Pediatrics, University of Illinois College of Medicine at Peoria and the Children's Hospital of Illinois at OSF Saint Francis Medical Center, Illinois, USA.
Department of Surgery, University of Illinois College of Medicine at Peoria and the Children's Hospital of Illinois at OSF Saint Francis Medical Center, Illinois, USA.
J Med Microbiol. 2011 Mar;60(Pt 3):317-322. doi: 10.1099/jmm.0.025841-0. Epub 2010 Nov 11.
The co-existence of multiple genotypes in colonization by Staphylococcus aureus has not been fully investigated. The aim of this study was to evaluate the heterogeneity of S. aureus carriage in children. We evaluated 125 nasal and perianal swab samples that were positive for S. aureus from 76 children scheduled for elective surgery. For each sample, at least four colonies with the same or different morphotypes were selected for analysis. Multiple-locus variable-number tandem-repeat fingerprinting was used to determine the genetic relatedness and to characterize the clonality of the S. aureus strains. Of the 125 swabs, 91 (73 %) contained meticillin-sensitive S. aureus (MSSA), 8 (6 %) contained meticillin-resistant S. aureus (MRSA), and 26 (21 %) contained MSSA and MRSA simultaneously. A total of 738 S. aureus strains were evaluated with a mean of 6 colonies (range 4-15) picked from each culture. Of the 125 swabs, 32 (26 %) samples contained two genetically distinct S. aureus strains and 6 (5 %) contained three different genotypes. Multiple S. aureus strains simultaneously carried by individual children were genetically unrelated to each other. We concluded that the co-existence of multiple genotypes of S. aureus was common. The significance of multiple carriage is yet to be determined, but this intraspecies interplay could be important to pathogenicity and virulence in S. aureus.
金黄色葡萄球菌定植中多种基因型的共存尚未得到充分研究。本研究旨在评估儿童金黄色葡萄球菌携带的异质性。我们评估了 76 名择期手术儿童的 125 份鼻和肛周拭子阳性的金黄色葡萄球菌样本。对于每个样本，至少选择了 4 个具有相同或不同形态的菌落进行分析。多位点可变数量串联重复指纹分析用于确定金黄色葡萄球菌菌株的遗传相关性和克隆性。在 125 个拭子中，91 个（73%）含有甲氧西林敏感金黄色葡萄球菌（MSSA），8 个（6%）含有耐甲氧西林金黄色葡萄球菌（MRSA），26 个（21%）同时含有 MSSA 和 MRSA。共评估了 738 株金黄色葡萄球菌，平均从每个培养物中挑选 6 个菌落（范围为 4-15）。在 125 个拭子中，32 个（26%）样本含有两种遗传上不同的金黄色葡萄球菌菌株，6 个（5%）样本含有三种不同的基因型。个体儿童同时携带的多种金黄色葡萄球菌菌株彼此之间没有遗传关系。我们得出结论，金黄色葡萄球菌多种基因型的共存很常见。多重携带的意义尚待确定，但这种种内相互作用可能对金黄色葡萄球菌的致病性和毒力很重要。
