转基因大豆MON89788事件特异性定量PCR方法的建立与评价
Establishment and evaluation of event-specific quantitative PCR method for genetically modified soybean MON89788.
作者信息
Takabatake Reona, Onishi Mari, Koiwa Tomohiro, Futo Satoshi, Minegishi Yasutaka, Akiyama Hiroshi, Teshima Reiko, Furui Satoshi, Kitta Kazumi
机构信息
Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki, Japan.
出版信息
Shokuhin Eiseigaku Zasshi. 2010;51(5):242-6. doi: 10.3358/shokueishi.51.242.
A novel real-time PCR-based analytical method was established for the event-specific quantification of a GM soybean event MON89788. The conversion factor (C(f)) which is required to calculate the GMO amount was experimentally determined. The quantitative method was evaluated by a single-laboratory analysis and a blind test in a multi-laboratory trial. The limit of quantitation for the method was estimated to be 0.1% or lower. The trueness and precision were evaluated as the bias and reproducibility of the relative standard deviation (RSD(R)), and the determined bias and RSD(R) values for the method were both less than 20%. These results suggest that the established method would be suitable for practical detection and quantification of MON89788.
建立了一种基于实时荧光定量PCR的新型分析方法，用于对转基因大豆事件MON89788进行事件特异性定量分析。通过实验确定了计算转基因生物量所需的换算因子（C(f)）。该定量方法通过单实验室分析和多实验室试验中的盲测进行评估。该方法的定量限估计为0.1%或更低。以相对标准偏差（RSD(R)）的偏差和重现性来评估准确性和精密度，该方法确定的偏差和RSD(R)值均小于20%。这些结果表明，所建立的方法适用于MON89788的实际检测和定量分析。
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