Zick Suzanna M, Ruffin Mack T, Djuric Zora, Normolle Daniel, Brenner Dean E
University of Michigan, Department of Family Medicine, 24 Frank Lloyd Wright Drive, P.O. Box 385, Lobby M, Ann Arbor, MI 48106, USA.
Int J Biomed Sci. 2010 Sep;6(3):233-240.
Zingiber officinale is one of the most commonly used spices. We developed a method to determine the main pungent ginger constituents, 6-, 8- and 10-gingerols and 6-shogaol in human plasma. Quantitation was achieved using a reversed-phase C(18) column using high-performance liquid chromatography with electrochemical detection. The assay was linear from 0.1 to 5.0 μg/mL. The within-day coefficients of variation for the assay at 5.0 μg/mL were ≤ 5% for all analytes. The recovery of all four analytes was ≥99% for at 5.0 μg/mL. The lower limit of quantitation was 0.1 μg/mL except for 10-gingerol which was 0.25 μg/mL. Currently, there is no analytical method for detecting pungent ginger constituents in human plasma. This HPLC method allows for the detection of all four of ginger's pungent constituents simultaneously in a relatively short run time of 25 minutes. This method should be useful for determining plasma levels of 6-, 8-, 10-gingerol and 6-shogaol in phase I clinical trials.
姜是最常用的香料之一。我们开发了一种方法来测定人血浆中主要的辛辣姜成分，即6-、8-和10-姜酚以及6-姜烯酚。使用反相C(18)柱，通过高效液相色谱结合电化学检测进行定量。该测定法在0.1至5.0μg/mL范围内呈线性。在5.0μg/mL时，所有分析物的日内变异系数≤5%。在5.0μg/mL时，所有四种分析物的回收率≥99%。除10-姜酚的定量下限为0.25μg/mL外，其余的定量下限均为0.1μg/mL。目前，尚无检测人血浆中辛辣姜成分的分析方法。这种高效液相色谱法能够在相对较短的25分钟运行时间内同时检测出姜的所有四种辛辣成分。该方法对于在I期临床试验中测定6-、8-、10-姜酚和6-姜烯酚的血浆水平应是有用的。
