Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Metallomics. 2010 Jun;2(6):400-6. doi: 10.1039/c003647a. Epub 2010 May 20.
Single metal ion-phospholipid complexes are observed in biphasic electrospray ionization mass spectrometry (BESI-MS) using a dual-channel microsprayer. Such a microsprayer makes it possible to put into contact two immiscible liquids within the Taylor cone. Thus, L-α-dipalmitoyl phosphatidylcholine (DPPC) dissolved in 1,2-dichloroethane (DCE) reacts with aqueous metal cations (M = Na(+), K(+), Ca(2+), Cu(2+), La(3+)) yielding the formation of M-DPPC(n) complexes. The number of phospholipid molecules ranges from 1 to 4 for monovalent ions, to 8 for divalent and to more than 10 for trivalent ions respectively. The large number of ligands observed involves the formation of solvent free single ion-phospholipid complexes.
在使用双通道微喷雾器的双相电喷雾电离质谱（BESI-MS）中观察到单金属离子-磷脂复合物。这种微喷雾器使得在泰勒锥内接触两种不混溶的液体成为可能。因此，溶解在 1,2-二氯乙烷（DCE）中的 L-α-二月桂酰基磷脂酰胆碱（DPPC）与水合金属阳离子（M=Na(+)、K(+)、Ca(2+)、Cu(2+)、La(3+)）反应生成[M-DPPC(n)]（z+）复合物。单价离子的磷脂分子数范围为 1 到 4，二价离子为 8，三价离子为 10 以上。观察到的大量配体涉及形成无溶剂的单离子-磷脂复合物。
