Department of Chemistry, Wayne State University, Detroit, Michigan, USA.
Metallomics. 2010 Jun;2(6):407-11. doi: 10.1039/c003414b. Epub 2010 May 6.
Polypyridyl ligands were investigated for their ability to bind and mobilize iron(ii) from ferritin in aqueous solution. Association constants with iron(ii) (pFe(II)) were determined for the pentadentate ligands N4Py (pFe(II) = 14.4) and Bn-TPEN (pFe(II) = 13.7) using a competition method with the hexadentate ligand TPEN (pFe(II) = 14.6, 0.1 M KNO(3)). Ferrous complexes were formed using the polypyridyl ligands and ferritin as the sole iron source in the presence of reductant. The observed rates of iron mobilization from ferritin were dependent on reductant and were higher in the presence of ascorbate than dithiothreitol. TPEN, N4Py and Bn-TPEN demonstrated comparable and in some cases faster rates and higher levels of iron mobilization when compared to the iron(ii) chelator 1,10-phenanthroline, particularly at low concentrations of chelator.
研究了多吡啶配体在水溶液中与铁蛋白结合并将铁（II）从铁蛋白中动员出来的能力。使用六齿配体 TPEN（pFe(II) = 14.6，0.1 M KNO(3)）作为竞争方法，确定了五齿配体 N4Py（pFe(II) = 14.4）和 Bn-TPEN（pFe(II) = 13.7）与铁（II）的结合常数（pFe(II)）。亚铁配合物是使用多吡啶配体在还原剂存在下，仅使用铁蛋白作为铁源形成的。从铁蛋白中动员铁的观察到的速率取决于还原剂，并且在存在抗坏血酸时比二硫苏糖醇时更高。与铁（II）螯合剂 1,10-邻菲啰啉相比，TPEN、N4Py 和 Bn-TPEN 表现出相当甚至在某些情况下更快的速度和更高的铁动员水平，特别是在较低浓度的螯合剂时。
