Human Protection and Performance Division, Defence Science and Technology Organisation, 506 Lorimer St, Fishermans Bend, Victoria 3207, Australia.
Rapid Commun Mass Spectrom. 2010 Dec 15;24(23):3419-24. doi: 10.1002/rcm.4789.
A method using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography/mass spectrometry (GC/MS) analysis has been developed to gain insight into the degradation of the chemical warfare agent sulfur mustard in solution. Specifically, the described approach simplifies the sample preparation for GC/MS analysis to provide a rapid determination of changes in sulfur mustard abundance. These results were found to be consistent with those obtained using liquid-liquid extraction (LLE) GC/MS. The utility of the described approach was further demonstrated by the investigation of the degradation process in a complex matrix with surfactant added to assist solvation of sulfur mustard. A more rapid reduction in sulfur mustard abundance was observed using the HS-SPME approach with surfactant present and was similar to results from LLE experiments. Significantly, this study demonstrates that HS-SPME can simplify the sample preparation for GC/MS analysis to monitor changes in sulfur mustard abundance in solution more rapidly, and with less solvent and reagent usage than LLE.
已经开发出一种使用顶空固相微萃取（HS-SPME）随后进行气相色谱/质谱（GC/MS）分析的方法，以深入了解化学战剂芥子气在溶液中的降解情况。具体来说，该方法简化了 GC/MS 分析的样品制备，可快速确定芥子气丰度的变化。这些结果与使用液液萃取（LLE）GC/MS 获得的结果一致。通过在添加表面活性剂以助溶芥子气的复杂基质中研究降解过程，进一步证明了该方法的实用性。在有表面活性剂存在的情况下，使用 HS-SPME 方法观察到芥子气丰度更快地减少，与 LLE 实验的结果相似。重要的是，这项研究表明 HS-SPME 可以简化 GC/MS 分析的样品制备，以更快地监测溶液中芥子气丰度的变化，与 LLE 相比，使用的溶剂和试剂更少。
