Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Anal Chem. 2010 Dec 15;82(24):10125-8. doi: 10.1021/ac102217e. Epub 2010 Nov 12.
Up to now, all commercial matrix-assisted laser desorption/ionization (MALDI) mass spectrometers still can not efficiently analyze very large biomolecules. In this work, we report the development of a novel MALDI ion trap mass spectrometer which can enrich biomolecular ions to enhance the detection sensitivity. A charge detector was installed to measure the large ions directly. With this design, we report the first measurement of IgM with the mass-to-charge ratio (m/z) at 980 000. In addition, quantitative measurements of the number of ions can be obtained. A step function frequency scan was first developed to get a clear signal in the m/z range from 200,000 to 1,000,000.
到目前为止，所有商用基质辅助激光解吸/电离（MALDI）质谱仪仍然不能有效地分析非常大的生物分子。在这项工作中，我们报告了一种新型 MALDI 离子阱质谱仪的开发，该质谱仪可以富集生物分子离子以提高检测灵敏度。安装了一个电荷检测器来直接测量大离子。通过这种设计，我们报告了第一个质量电荷比（m/z）为 980000 的 IgM 的测量。此外，可以获得定量测量的离子数量。首先开发了阶跃函数频率扫描，以在 200000 到 1000000 的 m/z 范围内获得清晰的信号。
