Genomics Research Center, Academia Sinica, Nankang District, Taipei, Taiwan.
Anal Chem. 2011 Nov 1;83(21):8273-7. doi: 10.1021/ac202083c. Epub 2011 Oct 3.
This study presents the first report on the development of a matrix-assisted laser desorption ionization (MALDI) linear ion trap mass spectrometer for large biomolecular ion detection by frequency scan. We designed, installed, and tested this radio frequency (RF) scan linear ion trap mass spectrometer and its associated electronics to dramatically extend the mass region to be detected. The RF circuit can be adjusted from 300 to 10 kHz with a set of operation amplifiers. To trap the ions produced by MALDI, a high pressure of helium buffer gas was employed to quench extra kinetic energy of the heavy ions produced by MALDI. The successful detection of the singly charged secretory immunoglobulin A ions indicates that the detectable mass-to-charge ratio (m/z) of this system can reach ~385 000 or beyond.
本研究首次报道了一种基于基质辅助激光解吸电离（MALDI）的线性离子阱质谱仪的发展，该质谱仪用于通过频率扫描检测大生物分子离子。我们设计、安装和测试了这种射频（RF）扫描线性离子阱质谱仪及其相关电子设备，以显著扩展待检测的质量范围。RF 电路可以通过一组运算放大器从 300 调整到 10 kHz。为了捕获 MALDI 产生的离子，采用了高压氦缓冲气体来猝灭 MALDI 产生的重离子的额外动能。成功检测到单电荷分泌型免疫球蛋白 A 离子表明，该系统的可检测质荷比（m/z）可达~385000 或更高。
