Department of Periodontology, Dental School, National and Kapodestrian University of Athens, Athens, Greece.
J Oral Pathol Med. 2011 Apr;40(4):345-51. doi: 10.1111/j.1600-0714.2010.00969.x. Epub 2010 Nov 15.
The pyogenic granuloma (PG) is a common localized hyperplastic lesion of the oral cavity. The purpose of the present study was to investigate the immunohistochemical expression of endothelial nitric oxide synthases (eNOS) and CD105/endoglin in oral PGs, to evaluate their involvement in the angiogenetic pathways of the lesion.
Ninety-three PGs were included in the study. Sixteen tumors were further sub-classified as pregnancy tumors (PT) and seventeen as pyogenic granulomas with fibrosis (PGFM). Immunohistochemical expression of eNOS and CD105/endoglin was quantified by computerized image analysis with a semi-automated system. Percentage of staining and number of objects (positive vessels) were recorded for each case.
Intense eNOS expression was seen in 92 of 93 lesions. A statistically significant association was found between eNOS percentage of staining/eNOS positive vascular spaces (objects) and age of the patients (9% increase per decade of life). Approximately 40% less eNOS positive objects were recorded in PGFM compared with PGs. Intense membranous CD105/endoglin expression was seen in all cases. The percentage of CD105/endoglin staining was statistically increased in PGs compared with PT. Approximately 40% less CD105/endoglin objects were found in PGFM compared with PGs; 56% more CD105/endoglin objects were found in tongue lesions, compared with gingival lesions. There was no statistically significant correlation considering percentage of staining and number of objects between CD105/endoglin and eNOS.
It is suggested that eNOS and CD105/endoglin are involved in the angiogenetic pathways of PG.
化脓性肉芽肿（PG）是口腔常见的局限性增生性病变。本研究旨在探讨口腔 PG 中内皮型一氧化氮合酶（eNOS）和 CD105/内皮糖蛋白的免疫组织化学表达，评估它们在病变的血管生成途径中的作用。
本研究纳入 93 例 PG。其中 16 例肿瘤进一步分为妊娠性肿瘤（PT）和 17 例纤维性化脓性肉芽肿（PGFM）。采用计算机图像分析半自动化系统对 eNOS 和 CD105/内皮糖蛋白的免疫组织化学表达进行定量分析。记录每个病例的染色百分比和阳性血管数。
93 例病变中 92 例 eNOS 表达强烈。eNOS 染色百分比/eNOS 阳性血管空间（对象）与患者年龄之间存在统计学显著相关性（每十年生活增加 9%）。PGFM 中记录的 eNOS 阳性对象约减少 40%。所有病例均可见强烈的膜性 CD105/内皮糖蛋白表达。PG 中 CD105/内皮糖蛋白染色百分比明显高于 PT。与 PG 相比，PGFM 中 CD105/内皮糖蛋白对象减少约 40%；与牙龈病变相比，舌病变中 CD105/内皮糖蛋白对象多 56%。考虑到染色百分比和对象数量，CD105/内皮糖蛋白与 eNOS 之间没有统计学显著相关性。
提示 eNOS 和 CD105/内皮糖蛋白参与 PG 的血管生成途径。
