Center for Biophotonics, University of California, Davis , Sacramento, California 95817, United States.
Anal Chem. 2014 Jul 1;86(13):6604-9. doi: 10.1021/ac5012188. Epub 2014 Jun 13.
We report the development of a rapidly modulated multifocal detection scheme that enables full Raman spectra (~500-2000 cm(-1)) from a 2-D focal array to be acquired simultaneously. A spatial light modulator splits a laser beam to generate an m × n multifocal array. Raman signals generated within each focus are projected simultaneously into a spectrometer and imaged onto a TE-cooled CCD camera. A shuttering system using different masks is constructed to collect the superimposed Raman spectra of different multifocal patterns. The individual Raman spectrum from each focus is then retrieved from the superimposed spectra with no crosstalk using a postacquisition data processing algorithm. This system is expected to significantly improve the speed of current Raman-based instruments such as laser tweezers Raman spectroscopy and hyperspectral Raman imaging.
我们报告了一种快速调制的多点检测方案的开发，该方案可实现从 2-D 焦点阵列同时获取完整的拉曼光谱（约 500-2000 cm(-1))。空间光调制器将激光束分裂成 m×n 多点阵列。在每个焦点内产生的拉曼信号被同时投射到光谱仪中，并成像到 TE 冷却 CCD 相机上。使用不同的掩模构建了一个快门系统，以收集不同多点模式的叠加拉曼光谱。然后，使用后采集数据处理算法从叠加光谱中检索每个焦点的单独拉曼光谱，而没有串扰。该系统有望显著提高基于激光镊子拉曼光谱和高光谱拉曼成像等当前拉曼仪器的速度。
