Institute of Life Science, Jiangsu University, Zhenjiang, Jiangsu, PR China.
FEBS Lett. 2010 Dec 1;584(23):4775-82. doi: 10.1016/j.febslet.2010.11.005. Epub 2010 Nov 10.
A study was performed to investigate the phylogenetic relationship among AAAH members and to statistically evaluate sequence conservation and functional divergence. In total, 161 genes were identified from 103 species. Phylogenetic analysis showed that well-conserved subfamilies exist. Exon-intron structure analysis showed that the gene structures of AAAH were highly conserved across some different lineage species, while some species-specific introns were also found. The dynamic distribution of ACT domain suggested one gene fusion event has occurred in eukaryota. Significant functional divergence was found between some subgroups. Analysis of the site-specific profiles revealed critical amino acid residues for functional divergence. This study highlights the molecular evolution of this family and may provide a starting point for further experimental verifications.
本研究旨在调查 AAAH 成员之间的系统发育关系，并对序列保守性和功能分化进行统计学评估。共从 103 个物种中鉴定出 161 个基因。系统发育分析表明存在保守的亚家族。外显子-内含子结构分析表明，AAAH 的基因结构在一些不同谱系的物种中高度保守，而在一些物种中也发现了特定的内含子。ACT 结构域的动态分布表明真核生物中发生了一次基因融合事件。一些亚组之间存在显著的功能分化。对特定位点的分析揭示了功能分化的关键氨基酸残基。本研究强调了该家族的分子进化，可为进一步的实验验证提供起点。
