Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China.
Eur J Obstet Gynecol Reprod Biol. 2011 Mar;155(1):69-74. doi: 10.1016/j.ejogrb.2010.10.017. Epub 2010 Nov 11.
Luteinizing hormone (LH) plays an important role in the development of ovarian cancer, and has been shown to inhibit apoptosis in ovarian cancer cells. Similarly, survivin is a molecule that has been shown to inhibit apoptosis in other types of cancer. Therefore, the aim of this study was to determine whether survivin can be induced by LH in ovarian cancer, and whether this induction influences the sensitivity of ovarian cancers to chemotherapy.
Survivin expression was monitored using western blot assays, and flow cytometry was used to detect the effects of cisplatin on the induction of apoptosis by LH. MTT assays were also used to analyze rates of cell proliferation.
Administration of LH in vitro induced survivin expression in a dose-dependent manner. Moreover, this signaling was dependent on the ERK1/2 signaling pathway. LH also blocked apoptosis induced by cisplatin.
These results suggest that LH influences the sensitivity of ovarian cancer cells to chemotherapy via signaling to inhibit apoptosis that also upregulates survivin.
黄体生成素（LH）在卵巢癌的发展中起着重要作用，并已被证明能抑制卵巢癌细胞凋亡。同样，存活素是一种已被证明能抑制其他类型癌症细胞凋亡的分子。因此，本研究旨在确定 LH 是否能诱导卵巢癌细胞中的存活素，以及这种诱导是否会影响卵巢癌对化疗的敏感性。
使用 Western blot 检测存活素的表达，并使用流式细胞术检测 LH 对顺铂诱导凋亡的影响。MTT 检测也用于分析细胞增殖率。
体外给予 LH 以剂量依赖的方式诱导存活素表达。此外，这种信号转导依赖于 ERK1/2 信号通路。LH 还阻断了顺铂诱导的凋亡。
这些结果表明，LH 通过信号转导抑制凋亡，同时上调存活素，影响卵巢癌细胞对化疗的敏感性。
