School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China.
Dev Dyn. 2011 Aug;240(8):2028-33. doi: 10.1002/dvdy.22693.
The synuclein family consists of three small intracellular proteins mainly expressed in neural tissues, and has been associated with human neurodegenerative diseases. We have examined the spatial and temporal expression patterns of three synuclein genes during embryogenesis of Xenopus laevis. The Xenopus synucleins were firstly expressed in the developing nervous system at the tail bud stages. At tadpole stages, Xenopus snca was expressed in the brain, branchial arch and somite, and sncbb signals were detected in entire brain and spinal cord. However, sncg was only expressed in the peripheral nervous system including trigeminal nerve and dorsal root ganglion. RT-PCR indicated that expression of synucleins was up-regulated at the end of neurulation, and then maintained at later examined stages. Our study provides the spatiotemporal expression patterns of the synuclein family genes in Xenopus embryos, and forms a basis for further functional analysis of synucleins.
突触核蛋白家族由三个主要在神经组织中表达的小细胞内蛋白组成，与人类神经退行性疾病有关。我们研究了非洲爪蟾（Xenopus laevis）胚胎发生过程中三种突触核蛋白基因的时空表达模式。非洲爪蟾的突触核蛋白首先在尾部芽期的发育中的神经系统中表达。在蝌蚪阶段，Xenopus snca 在大脑、鳃弓和体节中表达，而 sncbb 信号在整个大脑和脊髓中被检测到。然而，sncg 仅在包括三叉神经和背根神经节在内的周围神经系统中表达。RT-PCR 表明，突触核蛋白的表达在神经胚结束时上调，然后在以后检查的阶段保持不变。我们的研究提供了突触核蛋白家族基因在非洲爪蟾胚胎中的时空表达模式，为进一步分析突触核蛋白的功能奠定了基础。
