Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA.
Dev Cell. 2010 Nov 16;19(5):765-77. doi: 10.1016/j.devcel.2010.10.010.
Brassinosteroids (BRs) regulate a wide range of developmental and physiological processes in plants through a receptor-kinase signaling pathway that controls the BZR transcription factors. Here, we use transcript profiling and chromatin-immunoprecipitation microarray (ChIP-chip) experiments to identify 953 BR-regulated BZR1 target (BRBT) genes. Functional studies of selected BRBTs further demonstrate roles in BR promotion of cell elongation. The BRBT genes reveal numerous molecular links between the BR-signaling pathway and downstream components involved in developmental and physiological processes. Furthermore, the results reveal extensive crosstalk between BR and other hormonal and light-signaling pathways at multiple levels. For example, BZR1 not only controls the expression of many signaling components of other hormonal and light pathways but also coregulates common target genes with light-signaling transcription factors. Our results provide a genomic map of steroid hormone actions in plants that reveals a regulatory network that integrates hormonal and light-signaling pathways for plant growth regulation.
油菜素内酯（BRs）通过受体激酶信号通路调控植物的广泛发育和生理过程，该通路控制 BZR 转录因子。在这里，我们使用转录谱分析和染色质免疫沉淀微阵列（ChIP-chip）实验鉴定了 953 个 BR 调控 BZR1 靶标（BRBT）基因。对选定的 BRBT 的功能研究进一步证明了它们在 BR 促进细胞伸长中的作用。BRBT 基因揭示了 BR 信号通路与参与发育和生理过程的下游成分之间的许多分子联系。此外，结果还揭示了 BR 与其他激素和光信号通路在多个层面上的广泛串扰。例如，BZR1 不仅控制其他激素和光信号通路的许多信号成分的表达，还与光信号转录因子共同调节共同的靶基因。我们的研究结果提供了植物甾体激素作用的基因组图谱，揭示了一个整合激素和光信号通路的调控网络，用于植物生长调控。
