Institute of Molecular and Cell Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
Curr Opin Plant Biol. 2010 Feb;13(1):27-33. doi: 10.1016/j.pbi.2009.10.007. Epub 2009 Dec 7.
Large numbers of receptor-like kinases (RLKs) play key roles in plant development and defense by perceiving extracellular signals. The mechanisms of ligand-induced kinase activation and downstream signal transduction have been studied for only a few RLK pathways, among which the brassinosteroid (BR) pathway is the best characterized. Recently, proteomics studies identified new components that bridge the last gap in the genetically defined BR-signaling pathway, establishing the first complete pathway from an RLK to transcription factors in plants. Furthermore, analyses of phosphorylation events, mostly by mass spectrometry, provided insights into the mechanistic details of receptor kinase activation and regulation of downstream components by phosphorylation. This review focuses on recent progress in understanding BR signal transduction made by proteomics studies.
大量的受体样激酶（RLKs）通过感知细胞外信号在植物发育和防御中发挥关键作用。目前仅对少数几个 RLK 途径的配体诱导激酶激活和下游信号转导机制进行了研究，其中植物固醇（BR）途径是研究最为深入的途径。最近，蛋白质组学研究鉴定了新的成分，这些成分填补了遗传定义的 BR 信号转导途径中最后一个空白，从而在植物中建立了第一个从 RLK 到转录因子的完整途径。此外，通过质谱分析等方法对磷酸化事件进行分析，为受体激酶激活和磷酸化对下游成分调控的机制细节提供了深入了解。本文综述了蛋白质组学研究在理解 BR 信号转导方面的最新进展。
