Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Ecotoxicol Environ Saf. 2011 May;74(4):741-7. doi: 10.1016/j.ecoenv.2010.10.034. Epub 2010 Nov 12.
We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96 h of exposure to increasing concentrations of the herbicide (0-70 mg L(-1)) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96 h 55.62 mg L(-1)). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70 mg L(-1). Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri.
我们研究了一种草甘膦制剂的毒性，并提供了证据表明，由于暴露于除草剂而导致的藻类耐受力的氧化应激，会引起代谢改变。在用烷基芳基聚乙二醇醚表面活性剂处理藻类 96 小时后，藻类对除草剂的浓度（0-70mg/L）呈上升趋势，生长受到抑制（96 小时 EC50-55.62mg/L）。草甘膦增加了蛋白质和丙二醛的含量，在 50-70mg/L 时明显高于对照组。超氧化物歧化酶和过氧化氢酶的活性以及还原型谷胱甘肽的水平呈浓度依赖性增加。形态学研究表明，空泡化和细胞及孢子的大小增加。研究中的草甘膦制剂通过诱导氧化应激对 C. kessleri 具有细胞毒性作用。在草甘膦暴露后，SOD 和 CAT 活性以及 MDA 水平等氧化应激参数可能比通常在 C. kessleri 中测试的生长参数更敏感的生物标志物。
