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Improved yields of long PCR products using gene 32 protein.

作者信息

Schwarz K, Hansen-Hagge T, Bartram C

机构信息

Department of Pediatrics II, University of Ulm, FRG.

出版信息

Nucleic Acids Res. 1990 Feb 25;18(4):1079. doi: 10.1093/nar/18.4.1079.

DOI:10.1093/nar/18.4.1079
PMID:2107527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330396/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84fd/330396/74b5a1e7d023/nar00188-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84fd/330396/74b5a1e7d023/nar00188-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84fd/330396/74b5a1e7d023/nar00188-0368-a.jpg

相似文献

1
Improved yields of long PCR products using gene 32 protein.使用基因32蛋白提高长PCR产物的产量。
Nucleic Acids Res. 1990 Feb 25;18(4):1079. doi: 10.1093/nar/18.4.1079.
2
Colourimetric detection of PCR products using the DNA-binding protein TyrR.使用DNA结合蛋白TyrR对PCR产物进行比色检测。
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3
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Cloning of PCR products after defined cohesive termini are created with T4 DNA polymerase.用T4 DNA聚合酶产生特定粘性末端后对PCR产物进行克隆。
Nucleic Acids Res. 1990 Jul 25;18(14):4290. doi: 10.1093/nar/18.14.4290.
5
Laser cross-linking of protein-nucleic acid complexes.蛋白质-核酸复合物的激光交联
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A specific model for the conformation of single-stranded polynucleotides in complex with the helix-destabilizing protein GP32 of bacteriophage T4.
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A Raman spectroscopic study of the interaction between nucleotides and the DNA binding protein gp32 of bacteriophage T4.一项关于核苷酸与噬菌体T4的DNA结合蛋白gp32之间相互作用的拉曼光谱研究。
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Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes.用于聚合酶链反应扩增人免疫球蛋白可变基因的寡核苷酸引物及家族特异性寡核苷酸探针的设计。
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PCR dot blots: large signal differences between sense and anti-sense probes.
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An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.一种由T4基因32蛋白介导的改进的基于核酸序列的扩增方法。
PLoS One. 2022 Mar 24;17(3):e0265391. doi: 10.1371/journal.pone.0265391. eCollection 2022.
2
Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein.单链结合蛋白促进长的离散 DNA 片段的等温扩增。
Sci Rep. 2017 Aug 17;7(1):8497. doi: 10.1038/s41598-017-09063-x.
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Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 2. DNA diversity detected by PCR.

本文引用的文献

1
Products of bacteriophage T4 genes 32 and 45 improve the accuracy of DNA replication in vitro.
J Biol Chem. 1983 Oct 25;258(20):12274-9.
2
Primary structure of the bacteriophage T4 DNA helix-destabilizing protein.噬菌体T4 DNA解旋蛋白的一级结构。
J Biol Chem. 1981 Feb 25;256(4):1754-62.
3
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
小麦贮藏蛋白:以色列和土耳其野生二粒小麦(Triticum dicoccoides)中的醇溶蛋白多样性。2. 通过 PCR 检测到的 DNA 多样性。
Theor Appl Genet. 1995 Aug;91(3):409-14. doi: 10.1007/BF00222967.
4
Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein.利用T4噬菌体基因32蛋白优化体外转录和全长cDNA合成
J Biomol Tech. 2005 Sep;16(3):239-47.
5
Rapid detection of human pathogenic orthobunyaviruses.人类致病性正布尼亚病毒的快速检测
J Clin Microbiol. 2003 Jul;41(7):3299-305. doi: 10.1128/JCM.41.7.3299-3305.2003.
6
Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation.古菌dUTPase通过防止dUTP掺入,增强了古菌DNA聚合酶的PCR扩增效果。
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):596-601. doi: 10.1073/pnas.012372799. Epub 2002 Jan 8.
7
Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR.低模板浓度下逆转录酶(RT)对PCR的抑制作用及其对定量逆转录PCR的影响。
Appl Environ Microbiol. 1998 Feb;64(2):669-77. doi: 10.1128/AEM.64.2.669-677.1998.
8
Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein.使用牛血清白蛋白或T4基因32蛋白缓解PCR中的扩增抑制。
Appl Environ Microbiol. 1996 Mar;62(3):1102-6. doi: 10.1128/aem.62.3.1102-1106.1996.
9
Inactivation of tumor suppressor genes and deregulation of the c-myc gene in urothelial cancer cell lines.尿路上皮癌细胞系中肿瘤抑制基因的失活及c-myc基因的失调。
Urol Res. 1995;23(5):293-300. doi: 10.1007/BF00300017.
10
A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization.一种通过聚合酶链反应(PCR)和寡核苷酸探针杂交检测牡蛎中人类肠道病毒的病毒粒子浓缩方法。
Appl Environ Microbiol. 1996 Jun;62(6):2074-80. doi: 10.1128/aem.62.6.2074-2080.1996.