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PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.PCR诱导（无连接酶）亚克隆：一种快速可靠的亚克隆聚合酶链反应（PCR）产物的方法。
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2
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Cloning Polymerase Chain Reaction Products: Addition of Restriction Sites to the Termini of Amplified DNA.克隆聚合酶链反应产物：在扩增 DNA 的末端添加限制酶切位点。
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Exonuclease III induced ligase-free directional subcloning of PCR products.核酸外切酶III诱导的PCR产物无连接酶定向亚克隆
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Rapid isolation of a rice waxy sequence: a simple PCR method for the analysis of recombinant plasmids from intact Escherichia coli cells.水稻蜡质序列的快速分离：一种用于分析来自完整大肠杆菌细胞的重组质粒的简单PCR方法。
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Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.重叠延伸 PCR 克隆：一种创建重组质粒的简单可靠方法。
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Circular polymerase extension cloning of complex gene libraries and pathways.复杂基因文库和途径的环化聚合酶延伸克隆
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Competitive priming PCR: a versatile method to generate cohesive terminus.竞争性引物 PCR：一种生成粘性末端的通用方法。
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本文引用的文献

1
Direct cloning and sequence analysis of enzymatically amplified genomic sequences.酶促扩增基因组序列的直接克隆与序列分析
Science. 1986 Sep 5;233(4768):1076-8. doi: 10.1126/science.3461561.
2
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.DNA片段的体外制备及特异性诱变的通用方法：蛋白质与DNA相互作用的研究
Nucleic Acids Res. 1988 Aug 11;16(15):7351-67. doi: 10.1093/nar/16.15.7351.

PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.

作者信息

Shuldiner A R, Scott L A, Roth J

机构信息

Diabetes Branch, National Institutes of Health, Bethesda, MD 20892.

出版信息

Nucleic Acids Res. 1990 Apr 11;18(7):1920. doi: 10.1093/nar/18.7.1920.

DOI:10.1093/nar/18.7.1920
PMID:2186371
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330643/
Abstract
摘要