Department of Surgical and Radiological Sciences, University of California Davis, Davis, CA 95616, USA.
Res Vet Sci. 2012 Feb;92(1):66-75. doi: 10.1016/j.rvsc.2010.10.012. Epub 2010 Nov 13.
The aim of this study was to compare the osteogenic and proliferative potential of canine mesenchymal stromal cells (cMSCs) derived from bone marrow (BM-cMSCs) and adipose tissue (AT-cMSCs). Proliferation potential was determined under varying oxygen tensions (1%, 5%, and 21% O(2)). Effects of reduced oxygen levels on the osteogenic differentiation of AT-cMSCs were also investigated. AT-cMSCs proliferated at a significantly faster rate than BM-cMSCs, although both cell types showed robust osteogenic differentiation. Culture in 5% and 1% O(2) impaired proliferation in cMSC from both sources and osteogenic differentiation in AT-cMSCs. Our data suggests that AT-cMSCs might be more suitable for use in a clinical situation, where large cell numbers are required for bone repair, due to their rapid proliferation combined with robust osteogenic potential. Our data also suggests that the inhibitory effects of hypoxia on both cell proliferation and differentiation should be considered when using MSCs in a potentially hypoxic environment such as a fracture site.
本研究旨在比较源自骨髓（BM-cMSCs）和脂肪组织（AT-cMSCs）的犬间充质基质细胞（cMSCs）的成骨和增殖潜能。在不同氧张力（1%、5%和 21%O2）下测定增殖潜能。还研究了低氧水平对 AT-cMSCs 成骨分化的影响。尽管两种细胞类型均显示出强大的成骨分化能力，但 AT-cMSCs 的增殖速度明显快于 BM-cMSCs。在 5%和 1%O2 中培养会损害两种来源的 cMSC 的增殖和 AT-cMSCs 的成骨分化。我们的数据表明，由于 AT-cMSCs 具有快速增殖和强大的成骨潜能，因此在需要大量细胞用于骨修复的临床情况下，可能更适合使用。我们的数据还表明，在骨折部位等潜在缺氧环境中使用 MSC 时，应考虑低氧对细胞增殖和分化的抑制作用。
