Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2435-2438. doi: 10.1099/ijs.0.027847-0. Epub 2010 Nov 12.
Five strains (Mizu2-1(T), Gon2-7, Koba6-1, Koyu2-2 and Miya2-2) of lactic acid bacteria (LAB) were isolated from flowers in Oze National Park, Japan, using anaerobic cultivation. The five isolates were found to share identical 16S rRNA gene sequences. The isolates exhibited low levels of 16S rRNA gene sequence similarity to known LAB; the closest recognized relatives of strain Mizu2-1(T) were the type strains of Lactobacillus kunkeei (94.9 %), Lactobacillus kefiri (94.1 %) and Lactobacillus buchneri (93.9 %). Comparative analyses of rpoA and pheS gene sequences demonstrated that the novel isolates did not show significant relationships to other Lactobacillus species. The strains were Gram-stain-positive, catalase-negative and heterofermentative. Anaerobic growth was better than aerobic growth. The isolates utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose and fructose. On the basis of phenotypic characteristics and phylogenetic data, the isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus ozensis sp. nov. is proposed. The type strain is Mizu2-1(T) ( = JCM 17196(T) = DSM 23829(T)).
从日本大泽国立公园的花朵中，使用厌氧培养法分离到了五株（Mizu2-1(T)、Gon2-7、Koba6-1、Koyu2-2 和 Miya2-2）乳酸菌（LAB）。这五个分离株的 16S rRNA 基因序列完全相同。这些分离株与已知的 LAB 具有较低的 16S rRNA 基因序列相似性；Mizu2-1(T) 株的最接近的公认亲缘关系是 Lactobacillus kunkeei（94.9%）、Lactobacillus kefiri（94.1%）和 Lactobacillus buchneri（93.9%）的模式菌株。rpoA 和 pheS 基因序列的比较分析表明，新型分离株与其他乳杆菌属物种没有明显的关系。这些菌株为革兰氏阳性、过氧化氢酶阴性、异型发酵。厌氧生长优于需氧生长。分离株利用窄范围的碳水化合物作为碳源和能源，包括葡萄糖和果糖。基于表型特征和系统发育数据，这些分离株代表了乳杆菌属的一个新种，命名为 Lactobacillus ozensis sp. nov. 。其模式菌株为 Mizu2-1(T)（ = JCM 17196(T) = DSM 23829(T)）。
