Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Department of Applied Biology and Chemistry, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1356-1359. doi: 10.1099/ijs.0.022988-0. Epub 2010 Jul 2.
Five strains (Ryu1-2(T), Gon2-9, Ryu4-3, Nog8-1 and Aza1-1) of lactic acid bacteria were isolated from flowers in mountainous areas in Japan, Oze National Park, Iizuna mountain and the Nikko area. The five isolates were found to share almost identical (99.6-100 % similar) 16S rRNA gene sequences and were therefore deemed to belong to the same species. These isolates exhibited low levels of 16S rRNA gene sequence similarity to known lactic acid bacteria; the closest recognized relatives to strain Ryu1-2(T) were the type strains of Lactobacillus hilgardii (92.8 % similarity), Lactobacillus kefiri (92.7 %), Lactobacillus composti (92.6 %) and Lactobacillus buchneri (92.4 %). Comparative analyses of rpoA and pheS gene sequences demonstrated that the novel isolates did not show significant relationships to other Lactobacillus species. The strains were Gram-stain-positive, catalase-negative and homofermentative. The isolates utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose and fructose. On the basis of phenotypic characteristics and phylogenetic data, these isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus floricola sp. nov. is proposed. The type strain is Ryu1-2(T) ( = NRIC 0774(T) = JCM 16512(T) = DSM 23037(T)).
五种乳酸菌菌株（Ryu1-2(T)、Gon2-9、Ryu4-3、Nog8-1 和 Aza1-1）从日本山区、大沼国家公园、饭沼山和日光地区的花卉中分离得到。这五个分离株的 16S rRNA 基因序列几乎完全相同（99.6-100%相似），因此被认为属于同一物种。这些分离株与已知的乳酸菌的 16S rRNA 基因序列相似度较低；与 Ryu1-2(T) 株最接近的是 Lactobacillus hilgardii（92.8%相似）、Lactobacillus kefiri（92.7%）、Lactobacillus composti（92.6%）和 Lactobacillus buchneri（92.4%）的模式菌株。rpoA 和 pheS 基因序列的比较分析表明，新型分离株与其他 Lactobacillus 物种没有显著的关系。这些菌株革兰氏阳性、过氧化氢酶阴性且同型发酵。这些分离株利用窄范围的碳水化合物作为碳源和能源，包括葡萄糖和果糖。基于表型特征和系统发育数据，这些分离株代表了 Lactobacillus 属的一个新种，提议将其命名为 Lactobacillus floricola sp. nov.。模式株为 Ryu1-2(T)（=NRIC 0774(T)=JCM 16512(T)=DSM 23037(T)）。
