Department of Neurology, The Medical College of Wisconsin, 9000 W. Wisconsin Ave., PO Box 1997, Milwaukee, WI 53201, USA.
J Clin Neurophysiol. 2010 Dec;27(6):387-97. doi: 10.1097/WNP.0b013e3181fe06d8.
To test the hypothesis that focal and parafocal neocortical tissue from pediatric patients with intractable epilepsy exhibits cellular and synaptic differences, the authors characterized the propensity of these neurons to generate (a) voltage-dependent bursting and (b) synaptically driven paroxysmal depolarization shifts. Neocortical slices were prepared from tissue resected from patients with intractable epilepsy. Multiunit network activity and simultaneous whole-cell patch recordings were made from neurons from three patient groups: (1) those with normal histology; (2) those with mild and severe cortical dysplasia; and (3) those with abnormal pathology but without cortical dysplasia. Seizure-like activity was characterized by population bursting with concomitant bursting in intracellularly recorded cortical neurons (n = 59). The authors found significantly more N-methyl-D-aspartic acid-driven voltage-dependent bursting neurons in focal versus parafocal tissue in patients with severe cortical dysplasia (P < 0.01). Occurrence of paroxysmal depolarization shifts and burst amplitude and burst duration were significantly related to tissue type: focal or parafocal (P < 0.05). The authors show that functional differences between focal and parafocal tissue in patients with severe cortical dysplasia exist. There are functional differences between patient groups with different histology, and bursting properties can be significantly associated with the distinction between focal and parafocal tissue.
为了验证以下假设，作者测试了来自患有难治性癫痫的儿科患者的局灶性和旁灶性新皮质组织是否具有细胞和突触差异，即这些神经元是否具有产生(a)电压依赖性爆发和(b)突触驱动的阵发性去极化偏移的倾向。新皮质切片是从接受难治性癫痫手术切除的组织中制备的。作者对来自三个患者组的神经元进行了多单位网络活动和同时全细胞膜片钳记录：(1)组织学正常组；(2)轻度和重度皮质发育不良组；以及(3)异常病理但无皮质发育不良组。癫痫样活动的特征是群体爆发，同时伴有细胞内记录的皮质神经元爆发（n=59）。作者发现，在严重皮质发育不良患者中，局灶性与旁灶性组织中 N-甲基-D-天冬氨酸驱动的电压依赖性爆发神经元明显更多（P<0.01）。阵发性去极化偏移的发生以及爆发幅度和爆发持续时间与组织类型显著相关：局灶性或旁灶性（P<0.05）。作者表明，严重皮质发育不良患者局灶性和旁灶性组织之间存在功能差异。不同组织学患者组之间存在功能差异，爆发特性与局灶性和旁灶性组织之间的区别显著相关。
